Research Article

Wound Myiasis Caused by Sarcophaga (Liopygia) Argyrostoma (Robineau-Desvoidy) (Diptera: Sarcophagidae): Additional Evidences of the Morphological Identification Dilemma and Molecular Investigation

Table 1

Third larval stage of the most common Sarcophaga (Liopygia) species: comparison of the main morphological features, and related iconographic documentation, available in the literature.

Anatomical sitesSarcophaga (Liopygia) speciesOur findings
(III stage larvae)Sarcophaga argyrostomaSarcophaga crassipalpisSarcophaga cultellata(LM and SEM)

1st and 2nd thoracic segmentAt least anterior part of second thoracic segment without spines/warts on interband area [1, 10]NONEShape of the anterior spinose band of the first thoracic segment [3]Figures 1(a) and 1(b)

Anterior spiraclesTypal digit-like lobes with 10-11 lobes [17]Typal digit-like lobes with 12 lobes
[19]
Typal digit-like lobes with 15–18 lobes [3]Figure 1(c)

Posterior spiraclesPosterior spiracle, ovate or kidney-shaped
Ventral arch of the posterior
spiracle, long, reaching the inner arch area [17]
Posterior spiracle, round
Ventral arch of the posterior spiracle, short, not reaching the inner arch area
[19]
Posterior spiracle, kidney-shaped
A thick well sclerotized peritreme which is incomplete and divided into four regions: (a) a straight inner arch with a triangular protuberance at the ventral tip, (b) a scalloped shaped dorsal arch, (c) a round outer arch, (d) and a short and straight ventral arch. The inner arch is slightly oblique with respect to the sagittal plane of the larva, and the line drawn by this arch is reached by the free tip of ventral arch
Inner arch of posterior
spiracle oblique, almost parallel to the inner respiratory slit [3]
Figure 2

CephaloskeletonVentral cornua have a developed window
Ventral bridge of the intermediate sclerite with the tip truncated [17]
Ventral bridge of the intermediate sclerite with the tip rounded
[19]
Dorsal edge of the mouth hooks, including approximately two-thirds of the length of the tooth, being rectilinear and only curved at the tip
The parastomal bar is fused to the basal sclerite, and the anterior tip is usually pointing upward
(i) The window of the dorsal cornu is clearly 8-shaped
(ii) The dorsal bridge is more slender
(iii) The window of the ventral cornua is smaller [3]
Figures 3(a), 3(b), and 3(c)

Spiracular cavityEdge of spiracular cavity, highly scaly [17]NoneEdge of spiracular cavity slightly covered by filamentous spines [3]Figure 4