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The Scientific World Journal
Volume 2017, Article ID 9392532, 8 pages
Research Article

Antimutagenic Effect of Hibiscus sabdariffa L. Aqueous Extract on Rats Treated with Monosodium Glutamate

1Institute of Natural, Humanities and Social Sciences, Federal University of Mato Grosso, Sinop, MT, Brazil
2Department of Chemistry, Federal University of Piauí, Teresina, PI, Brazil
3Institute of Biological and Health Sciences, Federal University of Mato Grosso, Barra do Garças, MT, Brazil
4Institute of Health Sciences, Federal University of Mato Grosso, Sinop, MT, Brazil

Correspondence should be addressed to Ana Carla Guidini Valentini Gheller; moc.liamtoh@vgalracana

Received 11 October 2016; Revised 12 December 2016; Accepted 22 December 2016; Published 19 January 2017

Academic Editor: Paula B. Andrade

Copyright © 2017 Ana Carla Guidini Valentini Gheller et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Hibiscus sabdariffa L. is a plant of the Malvaceae family, commonly known as roselle. H. sabdariffa is known to contain antioxidant, cholesterol-lowering, antiobesity, insulin resistance reduction, antihypertensive, and skin cancer chemopreventive properties. This study evaluated the effects of H. sabdariffa aqueous extract against cyclophosphamide (CPA, 25 mg/Kg) induced damage to DNA in male Wistar rats by micronucleus test. Samples of H. sabdariffa calyx were obtained in the municipality of Barra do Garças, Mato Grosso, Brazil. The aqueous extract was prepared by infusion and each animal received a daily dose of 400 mg/Kg by gavage for 15 consecutive days of treatment. The presence of anthocyanins was confirmed by ferric chloride test and phenolic compounds using high-performance liquid chromatography, with emphasis on the identification of rutin. The animals were sacrificed by deepening of anaesthesia to obtain bone marrow and determination of the frequency of micronucleated polychromatic erythrocytes. The group treated with the aqueous extract of H. sabdariffa revealed a 91% reduction in micronucleus frequency when compared with the positive control group. Under the conditions tested, H. sabdariffa L. presented a protective effect to CPA-induced damage to DNA of the treated animals, and it is a potential candidate as a chemopreventive agent against carcinogenesis.