T-Bet Is Dependent on Stat-4 Inhibiting Acute Colitis but Not Stat-1 Using L4 Somatic Antigen of Heligmosomoides polygyrus

Figure 5

Relative gene expression of T-bet, RORγ-T, and Gata-3 from the colon of CAC models. Amplification was performed by gene-specific primers with 2 μL cDNA for 25 cycles (β-actin) or 35 cycles (transcription factor). (b) The fold change in T-bet mRNA (normalized to β-actin) expression in colon. (c) The fold change in RORT mRNA (normalized to β-actin) expression in colon. (d) The fold change in Gata-3 mRNA (normalized to β-actin) expression in colon. Results shown are mean±SD, with n=4 replicates in each group. p<0.05. p<0.001 (ns: not significant). Relative gene expression of p53 from the colon of CAC models. RNA was extracted from colons, and p53 gene expression was analyzed by real time PCR and normalized by β-actin mRNA. Results are representative of four independent experiments. Data with no statistically significant differences (ns: not significant).

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