Fecal Proteomic Analysis in Healthy Dogs and in Dogs Suffering from Food Responsive Diarrhea

Figure 1

2DE map of the feces proteins from healthy dog group and FRD dog group. The experiments were performed in triplicate for the two samples. Differently expressed protein spots are evidenced in red (see also Table 1 for protein identification). First dimension has been performed using an immobilized pH 3–10 linear gradient strip whereas the second dimension was a 13% SDS-PAGE. The standards were Bio-Rad low molecular weight (phosphorylase b, 97.4 kDa; bovine serum albumin, 66.2 kDa; ovalbumin 45.0 kDa; carbonic anhydrase, 31.0 kDa; soybean trypsin inhibitor, 21.5 kDa; lysozyme, 14.4 kDa).