RASGRP2 Suppresses Apoptosis via Inhibition of ROS Production in Vascular Endothelial Cells

Figure 1

Effect of RASGRP2 overexpression on Rap1 activation and NOX4 expression. (a) Expression of endogenous RASGRP2 protein with overexposure using X-ray film. 1: HUVEC, 2: TERT HUVEC. (b) Activated Rap1 in TERT HUVEC was recovered using RalGDS-RBD Agarose Beads and validated by western blot. M: mock cells; R: TERT HUVEC R cells. 1 and 2: each of two single cell clones obtained by establishment. (c) Expression of NOX4 protein in TERT HUVEC.