RASGRP2 Suppresses Apoptosis via Inhibition of ROS Production in Vascular Endothelial Cells

Figure 6

Effect of Rap1 siRNA on TNF-α stimulation. (a) Activated Rap1 in TERT HUVEC under Rap1 siRNA conditions was recovered using RalGDS-RBD agarose beads and validated by western blot. M: mock cells; R: TERT HUVEC R cells. (b) and (c) represent the cell viability of mock cells and TERT HUVEC R cells in TNF-α stimulation under condition Rap1 knocked down. Blue circle: control siRNA; red cross: control siRNA + TNF-α; green asterisk: Rap1 siRNA; purple square: Rap1 siRNA + TNF-α; n=3; A = P<0.01, Rap1 siRNA versus Rap1 siRNA + TNF-α; B = P<0.01, control siRNA + TNF-α versus Rap1 siRNA + TNF-α.

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