Coelomic Fluid of Lumbricus rubellus Synergistically Enhances Cytotoxic Effect of 5-Fluorouracil through Modulation of Focal Adhesion Kinase and p21 in HT-29 Cancer Cell Line

Figure 1

The combination therapy of CFL with 5-fluorouracil induced apoptosis in HT-29 cell line. (a) HT-29 cell line was cultured and divided into six groups: group 1 was without treatment (control); group 2 was treated with 5-fluorouracil, groups 3-5 were treated with either CFL 5, 10, or 20 µg/ml immediately after 5- fluorouracil, and group 6 was treated with CFL 20 µg/ml. The number of apoptosis cells showed that combination treatment has higher number than single treatment group, respectively (p<0.05; p<0.05; p<0.05). Red lines (isotype control). (b) Number of annexin-V positive cells indicated apoptosis cells. (c). Dose response of CFL showed cytotoxic synergistic effects (results shown are mean ± SD, with n =4 p < 0.05, p < 0.001.

(a)

(b)

(c)