Coelomic Fluid of Lumbricus rubellus Synergistically Enhances Cytotoxic Effect of 5-Fluorouracil through Modulation of Focal Adhesion Kinase and p21 in HT-29 Cancer Cell Line

Figure 3

The combination therapy of CFL with 5-fluorouracil reduced the expressions of Ca²⁺ intracellular level in HT-29 cells. (A) Control group. (B) Group was treated with 5FU only. (C), (D), and (E) HT-29 cells were treated with CFL 5, 10, and 20 g/mL, respectively, after 5-fluorouracil, (F) HT-29 cells were treated with CFL only. Green arrow: Ca²⁺ intracellular positive cell. All images were magnified at representative 4 independent experiments (G). Number of Ca²⁺ intracellular positive cells. The result of [iCa²⁺] expression also showed that combination groups have lower expression than 5FU only group, respectively (p<0.05; p<0.05; p<0.05). -value < 0.05; -value < 0.001.

(a)

(b)