Coelomic Fluid of Lumbricus rubellus Synergistically Enhances Cytotoxic Effect of 5-Fluorouracil through Modulation of Focal Adhesion Kinase and p21 in HT-29 Cancer Cell Line

Figure 4

CFL combined with 5-FU reduced p21 expressions of HT-29. The expression of p21 was assessed to evaluate the antimetastatic effect of CFL combined with 5-FU. (a) The percentage of p21 positive cells was analyzed using a FACS Calibur flow cytometer (BD Biosciences). Numbers represent the percentages of p21 positive cells were shown, orange lines (isotype control IgG2b). Results are representative of four independent experiments. (b) The percentage of p21 positive cells was shown inside the panels. Results shown are mean+SD, with n=4 replicates in each group. -value < 0.05; -value p< 0.001.

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