Research Article
Effect of Extraction Methods on Antioxidant Activity of Papery Skin Extracts and Fractions of Maja Cipanas Onion (Allium cepa L. var. ascalonicum)
Table 3
Antioxidant activity of sample to DPPH solution (n = 3).
| | Extraction method | Sample (n = 3) | Concentration (μg/mL) | % Scavenging | Linear equation | IC50 value (μg/mL) | AAI |
| | None | Ascorbic acid | 2 | 45.51 ± 0.03 | y = 6.52x + 33.00
R2 = 0.998 | 2.60 | 10.38 | | 4 | 59.12 ± 0.08 | | 6 | 72.90 ± 0.04 | | 8 | 85.74 ± 0.06 | | 10 | 97.45 ± 0.07 |
| | Maceration | Extract | 25 | 43.86 ± 0.04 | y = 0.083x + 42.86
R2 = 0.9966 | 86.28 | 0.31 | | 50 | 47.86 ± 0.05 | | 100 | 51.17 ± 0.03 | | 200 | 59.86 ± 0.08 | | 400 | 75.72 ± 0.06 | | Ethyl acetate fraction | 25 | 48.28 ± 0.06 | y = 0.083x + 47.11
R2 = 0.9975 | 34.92 | 0.77 | | | 50 | 52.00 ± 0.03 | | 100 | 55.31 ± 0.04 | | 200 | 64.00 ± 0.05 | | 400 | 79.87 ± 0.07 | | Water fraction | 25 | 37.10 ± 0.08 | y = 0.106x + 36.04 R2 = 0.9919 | 131.69 | 0.21 | | 50 | 40.97 ± 0.04 | | 100 | 47.86 ± 0.06 | | 200 | 59.03 ± 0.03 | | 400 | 77.38 ± 0.05 |
| | Percolation | Extract | 25 | 47.12 ± 0.05 | y = 0.067x + 46.12
R2 = 0.9907 | 55.62 | 0.49 | | 50 | 50.85 ± 0.03 | | 100 | 53.05 ± 0.06 | | 200 | 58.47 ± 0.04 | | 400 | 77.73 ± 0.07 | | Ethyl acetate fraction | 25 | 49.15 ± 0.04 | y = 0.069x + 47.08
R2 = 0.992 | 41.91 | 0.64 | | 50 | 50.85 ± 0.05 | | 100 | 54.41 ± 0.08 | | 200 | 59.32 ± 0.03 | | 400 | 75.59 ± 0.06 | | Water fraction | 25 | 34.07 ± 0.05 | y = 0.129x + 31.95
R2 = 0.993 | 139.82 | 0.19 | | 50 | 37.46 ± 0.04 | | 100 | 45.76 ± 0.08 | | 200 | 60.17 ± 0.03 | | 400 | 82.37 ± 0.07 |
| | Reflux method | Extract | 25 | 46.63 ± 0.03 | y = 0.064x + 45.02 R2 = 0.9934 | 78.07 | 0.35 | | 50 | 49.19 ± 0.05 | | 100 | 50.93 ± 0.04 | | 200 | 56.74 ± 0.07 | | 400 | 71.05 ± 0.08 | | Ethyl acetate fraction | 25 | 73.84 ± 0.05 | y = 0.066x + 47.95
R2 = 0.9923 | 31.31 | 0.86 | | 50 | 61.98 ± 0.03 | | 100 | 53.84 ± 0.04 | | 200 | 52.21 ± 0.08 | | 400 | 48.72 ± 0.06 | | Water fraction | 25 | 38.84 ± 0.08 | y = 0.089x + 37.94
R2 = 0.9919 | 134.88 | 0.20 | | 50 | 42.09 ± 0.07 | | 100 | 47.91 ± 0.04 | | 200 | 57.33 ± 0.05 | | 400 | 72.79 ± 0.03 |
| | Soxhlet method | Extract | 25 | 38.62 ± 0.09 | y = 0.116x + 37.64 R2 = 0.9929 | 107.08 | 0.25 | | 50 | 45.75 ± 0.06 | | 100 | 49.31 ± 0.03 | | 200 | 60.57 ± 0.04 | | 400 | 84.25 ± 0.05 | | Ethyl acetate fraction | 25 | 45.50 ± 0.09 | y = 0.081x + 43.40 R2 = 0.9955 | 84.06 | 0.32 | | 50 | 48.51 ± 0.05 | | 100 | 51.26 ± 0.07 | | 200 | 58.51 ± 0.04 | | 400 | 76.44 ± 0.03 | | 50 | 48.51 ± 0.05 | | Water fraction | 25 | 40.69 ± 0.07 | y = 0.086x + 39.13
R2 = 0.9948 | 126.05 | 0.21 | | 50 | 42.76 ± 0.04 | | 100 | 48.51 ± 0.03 | | 200 | 57.82 ± 0.05 | | 400 | 73.10 ± 0.06 |
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