The Efficacy of Processing Strategies on the Gastroprotective Potentiality of Chenopodium quinoa Seeds

Table 1

Oligonucleotide primer sequences used in conducting qRT-PCR of TNF-α and NF-kB gene expression protocol.

Gene	Forward primer (5′-3′)	Reverse primer (5′-3′)	Product number		bp
GAPDH	TTGTGCAGTGCCAGCCTCGT	TGCCGTTGAACTTGCCGTGG	NM_017008.4	60	201
TNF-α	TCTTCAAGGGACAAGGCTGC	CTTGATGGCAGAGAGGAGGC	NM_012675.3	60	104
NF-κB	CCCAAGTACCCGGATACAGC	GGGCAACTCATCTTCCGTGA	NM_001105720.2	60	124

GAPDH: glyceraldehyde-3-phosphate dehydrogenase (housekeeping gene), TNF-α: tumor necrosis factor alpha, NF-κB: nuclear factor kappa enhancer of activated B cells, : annealing temperature, bp: gene length in base pairs.