Determination of Cassiarin A Level of Cassia siamea Leaf Obtained from Various Regions in Indonesia Using the TLC-Densitometry Method

Table 1

Characteristics of Cassiarin A [18].

Method		Characteristic
1	Molecular weight	213.23 g/mol
2	RF value	0.34 ± 0.01 (silica GF254, chloroform : ethanol 85 : 15)
3	Color	Reddish solid
4	UV	λmax (EtOH) at 368 nm
5	IR	3420 cm^−1 and 2940 cm^−1 (characteristic peak of hydroxyl group (-OH) and/or (NH))
		1660 cm^−1 and 1620 cm^−1 (characteristic peak of ether)
		1395 cm^−1, 1370 cm^−1, and 1190 cm^−1
6	^1H-NMR position (numbering as in Figure 1)	Chemical shift (ppm)
	3	6.03 (1H, s)
	6	6.46 (1H, s)
	8	6.48 (1H, s)
	9	2.20 (3H, s)
	10	6.70 (1H, s)
	12	2.34 (3H, s)