Detection of Salmonella enterica subsp. enterica via Quenching of Unincorporated Amplification Signal Reporters in Loop-Mediated Isothermal Amplification

Figure 4

(a) Typical QUASR LAMP workflow in clinical diagnosis setting. The QUASR LAMP workflow is like the LAMP workflow (Figure 1) without the dye addition step. Not opening the tube after amplification makes QUASR LAMP less prone to contamination and false positives. (b) QUASR LAMP interpretation of results. Analysis can be done by viewing the reaction tubes through a blue LED light with a yellow filter. Positives appear bright fluorescent yellow green, and negatives appear nonfluorescent and dull.

(a)

(b)