Detection of Salmonella enterica subsp. enterica via Quenching of Unincorporated Amplification Signal Reporters in Loop-Mediated Isothermal Amplification

Table 2

SopD QUASR LAMP fluorophore-labeled primer and quencher probe set. The 5′ end of the FIP primer was appended with the FAM reporter dye (a green fluorophore). A short quencher probe complementary to the 5′ end of the labeled primer (FIPc) was manually designed. The quencher probe was modified at the 3′ end with a dark quencher (e.g., Black Hole quencher (BHQ)).

Label	Tm (°C)	GC content (%)	Length (bp)	Sequence (5′ ⟶ 3′)
SopD_S1_FIPc				6FAM/ACCTGCAGCTCATTCTGAGCAG-TCAAAAACAACGGCTCCGG
SopD_S1_FIPc_Q	36	50	12	TCCCGCAGAGG/BHQ1