Acute and Subacute Toxicity Assessment of Andrographolide-2-hydroxypropyl-β-cyclodextrin Complex via Oral and Inhalation Route of Administration in Sprague-Dawley Rats
Table 1
Evaluation of hematological parameters in repeated dose oral subacute toxicity study of AND-2-HyP-β-CYD complex.
Groups
Parameters
Normal control
Low dose (200 mg/kg)
Moderate dose (400 mg/kg)
High dose (666 mg/kg)
Reversal control
Reversal control of high dose (666 mg/kg)
Male
WBCB (×103 cells/μL)
2.36 ± 0.09
3.17 ± 0.29
2.40 ± 0.22
2.32 ± 0.26
1.53 ± 0.17
2.09 ± 0.14
RBC (×106 cells/μL
5.57 ± 0.09
5.43 ± 0.05
6.39 ± 0.31
7.00 ± 0.04
5.24 ± 0.40
6.93 ± 0.32
Mean HGB (g/dL)
12.88 ± 0.49
12.28 ± 0.51
12.70 ± 0.78
12.48 ± 0.59
9.25 ± 0.47
12.63 ± 0.59
HCT (%)
39.73 ± 1.7
39.83 ± 0.75
38.77 ± 1.97
39.93 ± 1.23
37.67 ± 1.41
39.00 ± 0.87
MCV (fL)
51.80 ± 0.45
54.83 ± 0.74
52.32 ± 1.49
52.48 ± 0.61
57.42 ± 2.38
54.80 ± 1.24
MCH (pg)
15.07 ± 0.22
16.13 ± 0.34
14.58 ± 0.44
14.00 ± 0.73
14.63 ± 0.54
14.47 ± 0.86
MCHC (g/dL)
28.95 ± 0.27
29.83 ± 0.59
30.50 ± 1.26
31.33 ± 0.67
32.03 ± 1.14
32.33 ± 0.99
PLT (×103 cells/μL)
928.67 ± 0.34
1062.33 ± 38.75
1042.83 ± 78.54
991.00 ± 54.04
1316.83 ± 61.02
1246.67 ± 42.52
NEUT (%)
11.56 ± 1.34
19.62 ± 1.96
17.92 ± 2.59
12.95 ± 0.84
18.00 ± 2.14
16.50 ± 2.30
LYM (%)
84.55 ± 1.74
76.72 ± 3.52
69.00 ± 7.06
81.03 ± 1.57
72.48 ± 3.20
68.92 ± 13.04
MONO (%)
3.57 ± 0.29
3.08 ± 0.14
3.38 ± 0.33
3.62 ± 0.24
3.95 ± 0.69
4.20 ± 0.62
EOS (%)
0.61 ± 0.10
0.43 ± 0.07
0.50 ± 0.07
0.65 ± 0.08
0.65 ± 0.09
1.08 ± 0.11
BASO (%)
0.57 ± 0.13
0.65 ± 0.14
0.38 ± 0.12
0.45 ± 0.10
0.42 ± 0.14
0.63 ± 0.09
Female
WBCB (×103 cells/μL)
2.51 ± 0.25
3.73 ± 0.35
1.96 ± 0.42
2.18 ± 0.30
1.53 ± 0.17
1.83 ± 0.15
RBC (×106 cells/μL
5.68 ± 0.16
5.47 ± 0.36
6.53 ± 0.13
7.18 ± 0.31
5.24 ± 0.40
5.97 ± 0.20
Mean HGB (g/dL)
10.35 ± 0.40
9.72 ± 0.32
9.87 ± 0.37
9.65 ± 0.24
9.25 ± 0.47
8.77 ± 0.27
HCT (%)
43.33 ± 2.14
43.67 ± 1.80
39.00 ± 1.65
42.27 ± 1.49
37.67 ± 1.41
45.33 ± 1.71
MCV (fL)
52.92 ± 0.67
54.75 ± 1.19
51.97 ± 0.31
50.72 ± 0.65
57.42 ± 2.38
56.15 ± 0.88
MCH (pg)
15.02 ± 0.37
16.43 ± 1.37
15.65 ± 0.88
15.00 ± 0.52
14.63 ± 0.54
14.88 ± 0.80
MCHC (g/dL)
28.72 ± 0.74
32.18 ± 2.45
31.67 ± 1.71
32.83 ± 0.65
32.03 ± 1.14
33.97 ± 1.48
PLT (×103 cells/μL)
927.00 ± 74.40
1127.67 ± 70.26
1191.00 ± 39.98
1286.33 ± 77.03
1316.83 ± 61.02
1130.00 ± 53.42
NEUT (%)
15.55 ± 1.76
23.80 ± 4.05
17.92 ± 2.36
15.68 ± 1.59
18.00 ± 2.14
15.00 ± 2.21
LYM (%)
77.30 ± 2.29
61.28 ± 6.58
70.70 ± 4.08
76.80 ± 2.43
72.48 ± 3.20
77.22 ± 3.61
MONO (%)
3.32 ± 0.39
3.33 ± 0.36
3.93 ± 0.32
4.25 ± 0.90
3.95 ± 0.69
3.45 ± 0.35
EOS (%)
0.42 ± 0.10
0.53 ± 0.11
0.65 ± 0.06
0.58 ± 0.09
1.15 ± 0.26
0.70 ± 0.07
BASO (%)
0.58 ± 0.12
0.55 ± 0.10
0.52 ± 0.08
0.42 ± 0.12
0.42 ± 0.14
0.60 ± 0.10
Each value represents the mean ± standard deviation (n = 6). One-way ANOVA test () followed by Dunnett’s test. significantly different.
