Diversity and Antimicrobial Activities of Actinobacteria Isolated from Mining Soils in Midelt Region, Morocco

Figure 5

Neighbor-joining phylogenetic tree based on 16S rRNA gene sequences of selected strains (antagonistic strains with high antimicrobial activity and other inactive strains). Blue, orange, purple, and pink circles represent strains from, respectively, Ahouli, Mibladen, Midelt, and Zaida sites. Red square represents GenBank closest type species. Number at branches designates bootstrap values of neighbor-joining method from 1000 replicates and the bar designates 1% sequence divergence. I and II: clades I and II; Ia, Ib, Ic, Id, and Ie: subclades Ia, Ib, Ic, Id and Ie; IIa, IIb, and IIc: subclades IIa, IIb, and IIc. The evolutionary distances were calculated using the maximum composite likelihood method, and the analysis was conducted in MEGA11 [31, 32].