Table of Contents
Ulcers
Volume 2013 (2013), Article ID 150780, 9 pages
http://dx.doi.org/10.1155/2013/150780
Research Article

Antioxidant Capacity, Cytoprotection, and Healing Actions of the Leaf Aqueous Extract of Ocimum suave in Rats Subjected to Chronic and Cold-Restraint Stress Ulcers

1Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon
2Department of Animal Biology, Higher Teacher's Training College, University of Yaoundé I, Yaoundé, Cameroon
3Department of Biomedical Sciences, Faculty of Health Sciences, University of Buea, Buea, Cameroon
4Institute of Medical Research and Study of Medicinal Plants (IMPM), Yaoundé, Cameroon

Received 24 November 2012; Accepted 1 March 2013

Academic Editor: Gyula Mozsik

Copyright © 2013 Paul V. Tan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. P. Bugard, “Modern life and gastric stress,” Monograph no. 893621/08-90, Imprimerie Le Brun, Caen, France, 1989.
  2. K. Nishida, Y. Ohta, T. Kobayashi, and I. Ishiguro, “Involvement of the xanthine-xanthine oxidase system and neutrophils in the development of acute gastric mucosal lesions in rats with water immersion restraint stress,” Digestion, vol. 58, no. 4, pp. 340–351, 1997. View at Google Scholar · View at Scopus
  3. A. S. Salim, “A possible new approach to the problem of refractory peptic ulceration. A role for free radical scavengers?” Scottish Medical Journal, vol. 36, no. 1, pp. 19–20, 1991. View at Google Scholar · View at Scopus
  4. F. Thonner, Flowering Plants of Africa, Weldon and Wesley, New York, NY, USA, 1915, Reprinted 1962 by Cramer and Hafner.
  5. W. C. Burger, “Families of flowering plants in Ethiopia. An Oklahoma State University USAID contract publication,” Experimental Bulletin, vol. 45, p. 117, 1967. View at Google Scholar
  6. J. M. Watt, M. Gerdinat, and K. Breyer-Brandwij, Medicinal and Poisonous Plants of Southern and Eastern Africa, E&S Livingstone, London, UK, 2nd edition, 1915.
  7. A. Bouquet, “Féticheurs et Médicine Traditionnelles au Congo (Brazzaville),” Memoire O.R.S.T.O.M. no. 36, Paris, France, 1969.
  8. J. Raynal, G. Troupin, and P. Sita, Flore et Médicine Traditionnelles. Mission d’étude 1979 au Rwanda. Rapport présenté a l’Agence de Coopération Culturelle et Technique (ACCT), Paris, France, 1979.
  9. E. N. Mwangi, A. Hassanali, S. Essuman, E. Myandat, L. Moreka, and M. Kimondo, “Repellent and acaricidal properties of Ocimum suave against Rhipicephalus appendiculatus ticks,” Experimental and Applied Acarology, vol. 19, no. 1, pp. 11–18, 1995. View at Publisher · View at Google Scholar · View at Scopus
  10. A. Seyoum, K. Pålsson, S. Kung'a et al., “Traditional use of mosquito-repellent plants in western Kenya and their evaluation in semi-field experimental huts against Anopheles gambiae: ethnobotanical studies and application by thermal expulsion and direct burning,” Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 96, no. 3, pp. 225–231, 2002. View at Publisher · View at Google Scholar · View at Scopus
  11. A. Seyoum, G. F. Killeen, E. W. Kabiru, B. G. J. Knols, and A. Hassanali, “Field efficacy of thermally expelled or live potted repellent plants against African malaria vectors in western Kenya,” Tropical Medicine and International Health, vol. 8, no. 11, pp. 1005–1011, 2003. View at Publisher · View at Google Scholar · View at Scopus
  12. E. Makonnen, A. Debella, D. Abebe, and F. Teka, “Analgesic properties of some Ethiopian medicinal plants in different models of nociception in mice,” Phytotherapy Research, vol. 17, no. 9, pp. 1108–1112, 2003. View at Publisher · View at Google Scholar · View at Scopus
  13. E. Makonnen, A. Debella, L. Zerihun, D. Abebe, and F. Teka, “Antipyretic properties of the aqueous and ethanol extracts of the leaves of Ocimum suave and Ocimum lamiifolium in mice,” Journal of Ethnopharmacology, vol. 88, no. 1, pp. 85–91, 2003. View at Publisher · View at Google Scholar · View at Scopus
  14. A. M. Janssen, J. J. C. Scheffer, L. Ntezurubanza, and A. Baerheim Svendsen, “Antimicrobial activities of some Ocimum species grown in Rwanda,” Journal of Ethnopharmacology, vol. 26, no. 1, pp. 57–63, 1989. View at Google Scholar · View at Scopus
  15. P. V. Tan, B. Nyasse, T. Dimo, and C. Mezui, “Gastric cytoprotective anti-ulcer effects of the leaf methanol extract of Ocimum suave (Lamiaceae) in rats,” Journal of Ethnopharmacology, vol. 82, no. 2-3, pp. 69–74, 2002. View at Publisher · View at Google Scholar · View at Scopus
  16. T. A. Miller, “Protective effects of prostaglandins against gastric mucosal damage: current knowledge and proposed mechanisms,” The American Journal of Physiology, vol. 245, no. 5, pp. G601–G623, 1983. View at Google Scholar · View at Scopus
  17. J. Bruneton, Phytochemistry and Pharmacognosy of Medicinal Plants: Technics, Lavoisier, 2nd edition, 1993.
  18. Y. Ohta, T. Kobayashi, K. Nishida, M. Nagata, and I. Ishiguro, “Therapeutic effect of Oren-gedoku-to extract on stress-induced acute gastric mucosal lesions in rats,” Phytotherapy Research, vol. 13, no. 7, pp. 588–592, 1999. View at Google Scholar
  19. N. R. Pillai and G. Santhakumari, “Effects of nimbidin on acute and chronic gastro-duodenal ulcer models in experimental animals,” Planta Medica, vol. 50, no. 2, pp. 143–146, 1984. View at Google Scholar · View at Scopus
  20. V. L. Singleton, R. Orthofer, and R. M. Lamuela-Raventós, “Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent,” Methods in Enzymology, vol. 299, pp. 152–178, 1998. View at Publisher · View at Google Scholar · View at Scopus
  21. I. F. F. Benzie and J. J. Strain, “The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay,” Analytical Biochemistry, vol. 239, no. 1, pp. 70–76, 1996. View at Publisher · View at Google Scholar · View at Scopus
  22. T. Hatano, H. Kagawa, T. Yasuhara, and T. Okuda, “Two new flavonoids and other constituents in licorice root: their relative astringency and radical scavenging effects,” Chemical and Pharmaceutical Bulletin, vol. 36, no. 6, pp. 2090–2097, 1988. View at Google Scholar · View at Scopus
  23. K. M. Wilbur, F. Bernheim, and O. W. Shapiro, “Determination of lipid peroxidation,” Archives of Biochemistry and Biophysics, vol. 24, pp. 305–310, 1949. View at Google Scholar
  24. G. L. Ellman, “Tissue sulfhydryl groups,” Archives of Biochemistry and Biophysics, vol. 82, no. 1, pp. 70–77, 1959. View at Google Scholar · View at Scopus
  25. H. P. Misra and I. Fridovich, “The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase,” The Journal of Biological Chemistry, vol. 247, no. 10, pp. 3170–3175, 1972. View at Google Scholar · View at Scopus
  26. A. K. Sinha, “Colorimetric assay of catalase,” Annals of Biochemistry, vol. 47, pp. 389–394, 1972. View at Google Scholar
  27. W. A. Mersereau and E. J. Hinchey, “Effect of gastric acidity on gastric ulceration induced by hemorrhage in the rat, utilizing a gastric chamber technique,” Gastroenterology, vol. 64, no. 6, pp. 1130–1135, 1973. View at Google Scholar · View at Scopus
  28. K. G. Lambert and C. H. Kinsley, “Sex differences and gonadal hormones influence susceptibility to the activity-stress paradigm,” Physiology and Behavior, vol. 53, no. 6, pp. 1085–1090, 1993. View at Publisher · View at Google Scholar · View at Scopus
  29. K. Somasundaram and A. K. Ganguly, “Gastric mucosal protection during restraint stress in rats: alteration in gastric adherent mucus and dissolved mucus in gastric secretion,” Hepato-Gastroenterology, vol. 32, no. 1, pp. 24–26, 1985. View at Google Scholar · View at Scopus
  30. P. V. Tan, C. Mezui, and M. Boda, “Anti-Helicobacter and gastroduodenal cytoprotective actions of the leaf aqueous extract of Ocimum suave (Lamiaceae),” Journal of Medicinal Plants Research, vol. 5, no. 25, pp. 5958–5966, 2011. View at Google Scholar
  31. T. Coskun, I. Alican, B. C. Yegen, T. San, S. Cetinel, and H. Kurtel, “Cyclosporin A reduces the severity of cold-restraint-induced gastric lesions: role of leukocytes,” Digestion, vol. 56, no. 3, pp. 214–219, 1995. View at Google Scholar · View at Scopus
  32. A. S. Salim, “Removing oxygen-derived free radicals stimulates healing of ethanol-induced erosive gastritis in the rat,” Digestion, vol. 47, no. 1, pp. 24–28, 1990. View at Google Scholar · View at Scopus
  33. M. Itoh and P. H. Guth, “Superoxide radicals play a role in haemorrhagic shock-induced gastric lesions in the rat,” Gastroenterology, vol. 82, p. 1122, 1984. View at Google Scholar
  34. A. S. Salim, “Role of oxygen-derived free radicals in mechanism of acute and chronic duodenal ulceration in the rat,” Digestive Diseases and Sciences, vol. 35, no. 1, pp. 73–79, 1990. View at Publisher · View at Google Scholar · View at Scopus
  35. M. Kolomoets, I. V. Kuz’menko, L. A. Chernukhina, and E. P. Klimenko, “Role of alpha tocopherol and retinol in the antiradical protection of the body in peptic ulcer,” Ukraïns’khyï Biokhimichnyï Zhurnal (Kiev), vol. 64, no. 2, pp. 79–84, 1992. View at Google Scholar
  36. T. Ohara, S. Asaki, and T. Toyota, “Biological evaluation of extracellular superoxide dismutase observed in a state of acute gastric mucosal lesions,” Japanese Journal of Gastroenterology, vol. 87, no. 1, pp. 1–7, 1990. View at Google Scholar · View at Scopus
  37. S. Mancinelli, G. de la Fuente, V. Manríquez et al., “The etiopathogenesis of the acute stress ulcer. The role of oxygen free radicals,” Revista Medica de Chile, vol. 118, no. 9, pp. 965–970, 1990. View at Google Scholar · View at Scopus
  38. R. Tandon, H. D. Khanna, M. Dorababu, and R. K. Goel, “Oxidative stress and antioxidants status in peptic ulcer and gastric carcinoma,” Indian Journal of Physiology and Pharmacology, vol. 48, no. 1, pp. 115–118, 2004. View at Google Scholar · View at Scopus
  39. A. S. Salim, “Oxygen-derived free radicals and the prevention of duodenal ulcer relapse: a new approach,” American Journal of the Medical Sciences, vol. 300, no. 1, pp. 1–8, 1990. View at Google Scholar · View at Scopus
  40. J. Javanmardi, C. Stushnoff, E. Locke, and J. M. Vivanco, “Antioxidant activity and total phenolic content of Iranian Ocimum accessions,” Food Chemistry, vol. 83, no. 4, pp. 547–550, 2003. View at Publisher · View at Google Scholar · View at Scopus
  41. A. C. Akinmoladun, E. O. Ibukun, E. Afor et al., “Chemical constituents and antioxidant activity of Alstonia boonei,” African Journal of Biotechnology, vol. 6, no. 10, pp. 1197–1201, 2007. View at Google Scholar · View at Scopus
  42. I. Klimczak, M. Malecka, M. Szlachta, and A. Gliszczynska-Swiglo, “Effect of storage on the contenent of polyphénols, vitamin C and the antioxidant activity of orange juices,” Al Niepodl, vol. 10, pp. 960–967, 2006. View at Google Scholar
  43. C. A. Rice-Evans, N. J. Miller, and G. Paganga, “Structure-antioxidant activity relationships of flavonoids and phenolic acids,” Free Radical Biology and Medicine, vol. 20, no. 7, pp. 933–956, 1996. View at Publisher · View at Google Scholar · View at Scopus
  44. A. Favier, “Le stress oxydant : Intérêt conceptuel et expérimental dans la compréhension des mécanismes des maladies et potentiel thérapeutique,” L' Actualité Chimique, no. 11-12, pp. 108–115, 2003. View at Google Scholar
  45. M. G. L. Hertog, E. J. M. Feskens, P. C. H. Hollman, M. B. Katan, and D. Kromhout, “Dietary antioxidant flavonoids and risk of coronary heart disease: The Zutphen Elderly Study,” The Lancet, vol. 342, no. 8878, pp. 1007–1011, 1993. View at Publisher · View at Google Scholar · View at Scopus
  46. S. Amrani, H. Harnafi, N. E. H. Bouanani et al., “Hypolipidaemic activity of aqueous Ocimum basilicum extract in acute hyperlipidaemia induced by triton WR-1339 in rats and its antioxidant property,” Phytotherapy Research, vol. 20, no. 12, pp. 1040–1045, 2006. View at Publisher · View at Google Scholar · View at Scopus
  47. P. V. Tan, C. Mezui, G. Enow-Orock, T. Dimo, and B. Nyasse, “Healing effect on chronic gastric ulcers and short-term toxicity profile of the leaf methanol extract of Ocimum suave(Lamiaceae) in rats,” African Journal of Traditional, Complimentary and Alternative Medicine, vol. 2, no. 3, pp. 312–325, 2005. View at Google Scholar
  48. O. Pascu and D. Dejica, “Oxygen free radicals and duodenal ulcer pain. Preliminary data,” Medecine Interne, vol. 25, no. 2, pp. 81–84, 1987. View at Google Scholar · View at Scopus
  49. P. V. Tan, B. Nyasse, G. E. Enow-Orock, P. Wafo, and E. A. Forcha, “Prophylactic and healing properties of a new anti-ulcer compound from Enantia chlorantha in rats,” Phytomedicine, vol. 7, no. 4, pp. 291–296, 2000. View at Google Scholar · View at Scopus