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Veterinary Medicine International
Volume 2010, Article ID 102591, 4 pages
http://dx.doi.org/10.4061/2010/102591
Research Article

Efficacy of Tuohy Needle in Oocytes Collection from Excised Mare Ovaries

1Università degli Studi di Milano, Large Animal Hospital, Reproduction Unit, via dell'Università 6, 26900 Lodi, Italy
2University of Wisconsin-River Falls, River falls, WI 54022, USA

Received 2 April 2010; Accepted 30 June 2010

Academic Editor: Sumanta Nandi

Copyright © 2010 F. Cremonesi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Two methods have been described to recover oocytes from equine follicles in excised ovaries: aspiration and scraping. Aim of this work was to develop an effective method for collecting equine oocytes using Tuohy needle and comparing this technique to aspiration and scraping, with or without tunica albuginea removal. This hollow hypodermic needle, usually employed for inserting epidural catheters, is designed with a slightly curved tip, shaped similar to a small curette. In unpeeled ovaries, the recovery rates of Tuohy needle group was higher ( 𝑃 < . 0 5 ) than in the 16 g needle aspiration and in the scraped ovaries (57% versus 36% and 47%) while the rate of cumulus-intact oocytes was higher than aspiration (46.9% versus 39.36%) but lower than scraping (46.97%) ( 𝑃 < . 0 0 1 ). In unpeeled ovaries there was significant difference in maturation rate of oocytes recovered by Tuohy needle in respect to peeled ovaries (58.54% versus 50.17%, resp.). Combination of aspiration and scraping by Tuohy needle allows a faster and reliable collection of oocytes suitable for horse IVM.