Efficacy of Tuohy Needle in Oocytes Collection from Excised Mare Ovaries

Table 1

Mean number of follicles and oocytes per ovary and recovery rate.

Collection technique	Follicles/ovary	Oocytes/ovary	recovery rate
			oocytes/follicles (%)
unpeeled and scraping	$6.04\mathrm{\hspace{0.17em}\hspace{0.17em}}\pm {2.27}^{\text{a}}$	$2.78\mathrm{\hspace{0.17em}\hspace{0.17em}}\pm \mathrm{\hspace{0.17em}\hspace{0.17em}}{0.94}^{\text{a}}$	$47\mathrm{\hspace{0.17em}\hspace{0.17em}}\pm \mathrm{\hspace{0.17em}\hspace{0.17em}}{0.02}^{\text{a}}$
peeled and scraping	$6.80\mathrm{\hspace{0.17em}\hspace{0.17em}}\pm \mathrm{\hspace{0.17em}\hspace{0.17em}}{0.20}^{\text{b}}$	$4.27\mathrm{\hspace{0.17em}\hspace{0.17em}}\pm {\mathrm{\hspace{0.17em}\hspace{0.17em}}0.30}^{\text{b}}$	$63\mathrm{\hspace{0.17em}\hspace{0.17em}}\pm \mathrm{\hspace{0.17em}\hspace{0.17em}}{0.03}^{\text{b}}$
unpeeled 16-g needle	$6.24\mathrm{\hspace{0.17em}\hspace{0.17em}}\pm \mathrm{\hspace{0.17em}\hspace{0.17em}}{0.85}^{\text{ab}}$	$\mathrm{\hspace{0.17em}\hspace{0.17em}}2.29\mathrm{\hspace{0.17em}\hspace{0.17em}}\pm \mathrm{\hspace{0.17em}\hspace{0.17em}}{0.45}^{\text{ab}}$	$36\mathrm{\hspace{0.17em}\hspace{0.17em}}\pm \mathrm{\hspace{0.17em}\hspace{0.17em}}{0.08}^{\text{c}}$
peeled 16-g needle	$8.21\mathrm{\hspace{0.17em}\hspace{0.17em}}\pm \mathrm{\hspace{0.17em}\hspace{0.17em}}{0.68}^{\text{c}}$	$3.16\mathrm{\hspace{0.17em}\hspace{0.17em}}\pm \mathrm{\hspace{0.17em}\hspace{0.17em}}{0.34}^{\text{c}\mathrm{\hspace{0.17em}\hspace{0.17em}}}$	$39\mathrm{\hspace{0.17em}\hspace{0.17em}}\pm {\mathrm{\hspace{0.17em}\hspace{0.17em}}0.01}^{\text{c}}$
unpeeled Tuohy needle	$5.76\mathrm{\hspace{0.17em}\hspace{0.17em}}\pm \mathrm{\hspace{0.17em}\hspace{0.17em}}{0.30}^{\text{a}}$	$\mathrm{\hspace{0.17em}\hspace{0.17em}}3.15\mathrm{\hspace{0.17em}\hspace{0.17em}}\pm \mathrm{\hspace{0.17em}\hspace{0.17em}}{0.76}^{\text{ad}}$	$57\mathrm{\hspace{0.17em}\hspace{0.17em}}\pm \mathrm{\hspace{0.17em}\hspace{0.17em}}{0.10}^{\text{d}}$
peeled Tuohy needle	$6.83\mathrm{\hspace{0.17em}\hspace{0.17em}}\pm \mathrm{\hspace{0.17em}\hspace{0.17em}}{1.15}^{\text{b}}$	$\mathrm{\hspace{0.17em}\hspace{0.17em}}3.67\mathrm{\hspace{0.17em}\hspace{0.17em}}\pm \mathrm{\hspace{0.17em}\hspace{0.17em}}{0.77}^{\text{be}}$	$56\mathrm{\hspace{0.17em}\hspace{0.17em}}\pm \mathrm{\hspace{0.17em}\hspace{0.17em}}{0.12}^{\text{d}}$

Values with different superscripts within the same column differ ($P<.05$).