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Veterinary Medicine International
Volume 2010 (2010), Article ID 274987, 7 pages
Research Article

Molecular Microbial Analysis of Lactobacillus Strains Isolated from the Gut of Calves for Potential Probiotic Use

Departamento de Salud Pública Veterinaria, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Kreder 2805 (S3080HOF) Esperanza, Santa Fe, Argentina

Received 1 June 2009; Accepted 29 July 2009

Academic Editor: Alessandro Mannelli

Copyright © 2010 Lorena P. Soto et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. S. Salminem and A. von Wright, Lactic Acid Bacteria: Microbiology and Functional Aspects, Marcel Dekker, Nueva York, NY, USA, 2nd edition, 1998.
  2. M. Rosmini, G. Sequeira, I. Guerrero-Legarreta et al., “Producción de probióticos para animales de abasto: importancia del uso de la microbiota intestinal indígena,” Revista Mexicana de Ingeniería Quimica, vol. 3, pp. 187–197, 2004. View at Google Scholar
  3. A. L. McCartney, “Application of molecular biological methods for studing probiotics and the gut flora,” British Journal of Nutrition, vol. 88, supplement 1, pp. S29–S37, 2002. View at Google Scholar
  4. S. Silvi, M. C. Verdenelli, C. Orpianesi, and A. Cresci, “EU project Crownalife: functional foods, gut microflora and healthy ageing: isolation and identification of Lactobacillus and Bifidobacterium strains from faecal samples of elderly subjects for a possible probiotic use in functional foods,” Journal of Food Engineering, vol. 56, no. 2-3, pp. 195–200, 2003. View at Publisher · View at Google Scholar
  5. H. G. H. J. Heilig, E. G. Zoetendal, E. E. Vaughan, P. Marteau, A. D. L. Akkermans, and W. M. de Vos, “Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA,” Applied and Environmental Microbiology, vol. 68, no. 1, pp. 114–123, 2002. View at Publisher · View at Google Scholar
  6. C. J. Ziemer, M. A. Cotta, and T. R. Whitehead, “Application of group specific amplified rDNA restriction analysis to characterize swine fecal and manure storage pit samples,” Anaerobe, vol. 10, no. 4, pp. 217–227, 2004. View at Publisher · View at Google Scholar
  7. R. Schneider, M. Rosmini, M. Ehrmann, and R. Vogel, “Identificación de bacterias lácticas componentes de la microbiota típica de los terneros criados en condiciones artificiales,” Revista FAVE—Ciencias Veterinarias, vol. 3, no. 1-2, pp. 7–15, 2004, (Argentina). View at Google Scholar
  8. L. L. Guan, K. E. Hagen, G. W. Tannock, D. R. Korver, G. M. Fasenko, and G. E. Allison, “Detection and identification of Lactobacillus species in crops of broilers of different ages by using PCR-denaturing gradient gel electrophoresis and amplified ribosomal DNA restriction analysis,” Applied and Environmental Microbiology, vol. 69, no. 11, pp. 6750–6757, 2003. View at Publisher · View at Google Scholar
  9. FAO/WHO, “Evaluation of health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. Expert consultation report,” Food and Agriculture Organization of the United Nations and World Health Organization, Córdoba, Argentina, October 2001.
  10. L. Soto, L. Frizzo, E. Bertozzi et al., “Milk evaluation as growth and cold preservation medium of a probiotic inoculum for young calves,” Journal of Animal and Veterinary Advances, vol. 8, no. 7, pp. 1353–1360, 2009. View at Google Scholar
  11. H. M. Timmerman, C. J. M. Koning, L. Mulder, F. M. Rombouts, and A. C. Beynen, “Monostrain, multistrain and multispecies probiotics: a comparison of functionality and efficacy,” International Journal of Food Microbiology, vol. 96, no. 3, pp. 219–233, 2004. View at Publisher · View at Google Scholar
  12. C. E. Morris, M. Bardin, O. Berge et al., “Microbial biodiversity: approaches to experimental design and hypothesis testing in primary scientific literature from 1975 to 1999,” Microbiology and Molecular Biology Reviews, vol. 66, no. 4, pp. 592–616, 2002. View at Publisher · View at Google Scholar
  13. C. M. Lee, C. C. Sieo, C. M. V. L. Wong, N. Abdullah, and Y. W. Ho, “Sequence analysis of 16S rRNA gene and 16S-23S rRNA gene intergenic spacer region for differentiation of probiotics Lactobacillus strains isolated from the gastrointestinal tract of chicken,” Annals of Microbiology, vol. 58, no. 1, pp. 133–140, 2008. View at Google Scholar
  14. G. Tannock, “Molecular assessment of intestinal microflora,” American Journal of Clinical Nutrition, vol. 73, no. 2, supplement, pp. 410S–414S, 2001. View at Google Scholar
  15. M. Ventura, M. Elli, R. Reniero, and R. Zink, “Molecular microbial analysis of Bifidobacterium isolates from different environments by the species-specific amplified ribosomal DNA restriction analysis (ARDRA),” FEMS Microbiology Ecology, vol. 36, no. 2-3, pp. 113–121, 2001. View at Publisher · View at Google Scholar
  16. V. Hall, T. Lewis-Evans, and B. I. Duerden, “Identification of actinomyces, propionibacteria, lactobacilli and bifidobacteria by amplified 16S rDNA restriction analysis,” Anaerobe, vol. 7, no. 2, pp. 55–57, 2001. View at Publisher · View at Google Scholar
  17. M. Kim and J. Chun, “Bacterial community structure in kimchi, a Korean fermented vegetable food, as revealed by 16S rRNA gene analysis,” International Journal of Food Microbiology, vol. 103, no. 1, pp. 91–96, 2005. View at Publisher · View at Google Scholar
  18. D. Roy, S. Sirois, and D. Vincent, “Molecular discrimination of lactobacilli used as starter and probiotic cultures by amplified ribosomal DNA restriction analysis,” Current Microbiology, vol. 42, no. 4, pp. 282–289, 2001. View at Publisher · View at Google Scholar
  19. J. Marmur, “A procedure for the isolation of deoxyribonucleic acid from microorganisms,” Journal of Molecular Biology, vol. 13, pp. 208–218, 1961. View at Google Scholar
  20. P. Kurzak, M. A. Ehrmann, and R. F. Vogel, “Diversity of lactic acid bacteria associated with ducks,” Systematic and Applied Microbiology, vol. 21, no. 4, pp. 588–592, 1998. View at Google Scholar
  21. G. Blaiotta, V. Fusco, D. Ercolini, M. Aponte, O. Pepe, and F. Villani, “Lactobacillus strain diversity based on partial hsp60 gene sequences and design of PCR-restriction fragment length polymorphism assays for species identification and differentiation,” Applied and Environmental Microbiology, vol. 74, no. 1, pp. 208–215, 2008. View at Publisher · View at Google Scholar
  22. L. Delfederico, A. Hollmann, M. Martínez, N. G. Iglesias, G. De Antoni, and L. Semorile, “Molecular identification and typing of lactobacilli isolated from kefir grains,” Journal of Dairy Research, vol. 73, no. 1, pp. 20–27, 2006. View at Publisher · View at Google Scholar
  23. P. Vandamme, B. Pot, M. Gillis, P. De Vos, K. Kersters, and J. Swings, “Polyphasic taxonomy, a consensus approach to bacterial systematics,” Microbiological Reviews, vol. 60, no. 2, pp. 407–438, 1996. View at Google Scholar
  24. S. Roos, F. Karner, L. Axelsson, and H. Jonsson, “Lactobacillus mucosae sp. nov., a new species with in vitro mucus-binding activity isolated from pig intestine,” International Journal of Systematic and Evolutionary Microbiology, vol. 50, no. 1, pp. 251–258, 2000. View at Google Scholar
  25. N. Thanantong, S. Edwards, and O. A. E. Sparagano, “Characterization of lactic acid bacteria and other gut bacteria in pigs by a macroarraying method,” Annals of the New York Academy of Sciences, vol. 1081, pp. 276–279, 2006. View at Publisher · View at Google Scholar
  26. Y.-J. Lee, W.-K. Yu, and T.-R. Heo, “Identification and screening for antimicrobial activity against Clostridium difficile of Bifidobacterium and Lactobacillus species isolated from healthy infant faeces,” International Journal of Antimicrobial Agents, vol. 21, no. 4, pp. 340–346, 2003. View at Publisher · View at Google Scholar
  27. M. Pascual, M. Hugas, J. I. Badiola, J. M. Monfort, and M. Garriga, “Lactobacillus salivarius CTC2197 prevents Salmonella enteritidis colonization in chickens,” Applied and Environmental Microbiology, vol. 65, no. 11, pp. 4981–4986, 1999. View at Google Scholar