Binding of Host Factors Influences Internalization and Intracellular Trafficking of Streptococcus uberis in Bovine Mammary Epithelial Cells
Figure 2
Effect of treatment with host proteins on receptor-mediated internalization of Streptococcus uberis into bovine mammary epithelial cells. Fibronectin (FN), collagen (Coll), and bovine lactoferrin (LF), pretreated S. uberis UT888 () and UT366 () were cocultured with bovine mammary epithelial cells treated with the caveolae-mediated endocytosis inhibitor Methyl-β−cyclodextran (MβC) and colony forming units per ml (cfu/mL) of intracellular S. uberis calculated. Data are presented as the percentage of untreated controls (100%; UT888 = 7.5 102; UT366 = 3.0 102) and bars represent the standard error of the mean (SEM) of three independent experiments run in triplicate. (*) indicates statistically significant differences () with the corresponding control. Means with similar superscript letters within each strain are statistically different ().
) and UT366 (
) were cocultured with bovine mammary epithelial cells treated with the caveolae-mediated endocytosis inhibitor Methyl-β−cyclodextran (MβC) and colony forming units per ml (cfu/mL) of intracellular S. uberis calculated. Data are presented as the percentage of untreated controls (100%; UT888 = 7.5 102; UT366 = 3.0 102) and bars represent the standard error of the mean (SEM) of three independent experiments run in triplicate. (*) indicates statistically significant differences () with the corresponding control. Means with similar superscript letters within each strain are statistically different ().
