Binding of Host Factors Influences Internalization and Intracellular Trafficking of Streptococcus uberis in Bovine Mammary Epithelial Cells
Figure 3
Effect of treatment with host proteins on transcytosis of Streptococcus uberis across bovine mammary epithelial cells treated with the receptor mediated endocytosis inhibitor monodansylcadaverine. Fibronectin (FN), collagen (Coll), or bovine lactoferrin (LF), pretreated S. uberis UT888 () and UT366 () were cocultured with bovine mammary epithelial cells treated with the receptor-mediated endocytosis inhibitor monodansylcadaverine (MDC), and colony forming units per ml (cfu/mL) of S. uberis that transcytosed bovine mammary epithelial cells were calculated at 60 minutes. Data are presented as the percentage of untreated controls (100%; UT888 = 6.0 102; UT366 = 3.0 102) and bars represent the standard error of the mean (SEM) of three independent experiments run in triplicate. (*) indicates statistically significant differences () with the corresponding control. Means with similar superscript letters within each strain are statistically different ().
) and UT366 (
) were cocultured with bovine mammary epithelial cells treated with the receptor-mediated endocytosis inhibitor monodansylcadaverine (MDC), and colony forming units per ml (cfu/mL) of S. uberis that transcytosed bovine mammary epithelial cells were calculated at 60 minutes. Data are presented as the percentage of untreated controls (100%; UT888 = 6.0 102; UT366 = 3.0 102) and bars represent the standard error of the mean (SEM) of three independent experiments run in triplicate. (*) indicates statistically significant differences () with the corresponding control. Means with similar superscript letters within each strain are statistically different ().
