Chronological Reorganization of Microtubules, Actin Microfilaments, and Chromatin during the First Cell Cycle in Swamp Buffalo (Bubalus bubalis) Embryos

Table 3

Percentage of maturation and fertilization of swamp buffalo oocytes at 12, 18, 24, and 30 h after IVF.

Time after IVF (h)	No. of oocytes (replicates)	No. of MII oocytes (%)*	No. of fertilization (%)**	Embryo development
				(% of fertilized oocytes)
				OA (%)	PF (%)	CC (%)
12	103 (9)	63 (61.2)	28.6a	5 (27.8)	13 (72.2)	0 (0)
18	97 (7)	65 (67.0)	50.8b	3 (9.1)	30 (90.9)	0 (0)
24	91 (7)	59 (64.9)	49.2b	4 (13.8)	25 (86.2)	0 (0)
30	87 (8)	56 (64.4)	48.2b	4 (14.9)	9 (33.3)	14 (51.8)

a,bWithin a column, differences were considered to be significant at 𝑃<.05. OA = oocyte activation; PF = pronuclear formation; CC = cell cleavage.
∗Percentage is expressed in relation to the total number of oocytes used for in vitro maturation.
∗∗Percentage is expressed in relation to the number of MII oocytes.