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Veterinary Medicine International
Volume 2010, Article ID 485060, 5 pages
http://dx.doi.org/10.4061/2010/485060
Research Article

The Immune Enhancement of Sodium Lauryl Sulfoacetate in Chickens

1College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China
2Department of Pharmacy, Jiangsu Animal Husbandry and Veterinary College, Taizhou 225300, China

Received 27 August 2009; Revised 10 January 2010; Accepted 2 March 2010

Academic Editor: Maurice Pensaert

Copyright © 2010 DaRong Cheng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The purpose of this study is to investigate feasibility of sodium lauryl sulfoacetate (SLS) as an immunoadjuvant in chickens. After treating with 62.5, 125, 250, or 500  𝜇 g/mL SLS in vitro, lymphocyte proliferation assay of chicken peripheral blood mononuclear cells showed that the O D 5 7 0 values of all experimental groups, as well as Con A-stimulated group, were significantly higher than that of the untreated control group. After injection with 1.0, 2.0, or 4.0 mg/kg of SLS for 3 consecutive days, chickens were vaccinated with an attenuated vaccine against Newcastle disease virus (NDV), and the immunoadjuvant effects of SLS were evaluated on the basis of immune organ index, antibody response, and C D 4 + / C D 8 + T-cell ratio. The results confirmed that SLS could enhance NDV-specific antibody response and increase C D 4 + / C D 8 + T-cell ratio in vivo. Furthermore, SLS could improve NDV-specific antibody response in thiamphenicol-treated chickens. These data indicate that SLS not only can improve humoral immune response but also reverse the immunosuppressive effect of thiamphenicol in chickens.