Veterinary Medicine International / 2010 / Article / Tab 1

Research Article

Stallion Sperm Viability, as Measured by the Nucleocounter SP-100, Is Affected by Extender and Enhanced by Single Layer Centrifugation

Table 1

Mean (±SD) values for sperm kinematics of cool-stored stallion spermatozoa in different extenders after Single Layer Centrifugation (n=45).

ParameterTimeUncentrifuged ICentrifuged IUncentrifuged KCentrifuged K

Total motility (%)0 hour76.4±12.4b83.8±8.9b74.8±12.477.1±14.3
24 hours63.0±15.5ab78.9±13.9b56.4±17.2ac65.1±19.7c

Progressive motility (%)0 hour54.7±17b67.3±16.5b52.6±15.756.8±18.2
24 hours40.2±16ab64.5±16.7b30.8±12.5ac42.7±21c

Velocity (μm/s)0 hours48.3±14.8b43.7±14.2b46.3±1440.6±13
24 hours38.1±15.6ab  49.6±14.5b28±11.5ac36.9±16.5c

Note: I:INRA96, K: Kenney extender. a: significant difference between INRA96 and Kenney’s extender for uncentrifuged samples; b: significant difference between uncentrifuged and centrifuged samples in INRA96; c: significant difference between uncentrifuged and centrifuged samples in Kenney’s extender.