Diagnostic Application of IS900 PCR Using Blood as a Source Sample for the Detection of Mycobacterium avium Subspecies Paratuberculosis in Early and Subclinical Cases of Caprine Paratuberculosis

Table 1

Evaluation of ELISA with blood-PCR and microscopic examination.

S/P ratios	Johne’s disease status	Number (%)	Positives
			Blood PCR	M${\text{E}}^{*}$
00.0–0.9	Negative	03 (14.3)	1	1
0.1–0.24	Suspected	00 (00.0)	—	—
0.25–0.39	Low Positive	06 (28.6)	2	5
0.4–0.9	Positive	10 (47.6)	5	7
1.0–10.0	Strong Positive	02 (09.5)	1	1
Total			9 (42.8)	14 (66.7)

${}_{}{}^{*}\text{M}$E: microscopic examination.