Review Article
Feline Neonatal Isoerythrolysis and the Importance of Feline Blood Types
Table 5
Crossmatching protocol. Major crossmatching should be compatible at
and
C (cold agglutinins) and minor at
C (Adapted from Fox [
41]).
| 1. Collect 2 mL of blood into EDTA from tom/kitten and queen. |
| 2. Centrifuge 1 minute, separate plasma from red blood cells. Keep plasma. |
| 3. Wash red blood cells two times, into at least twice its volume, with isotonic saline solution. | Discard supernatant and keep red blood cells. |
| 4. Dilute red blood cells at washed red blood cells plus 490 L isotonic saline solution. |
| 5. Major crossmatching: | 2 drops of (50 L) tom/kitten’s red blood cell dilution | 2 drops of (50 L) queen's plasma |
| 6. Minor crossmatching: | 2 drops of (50 L) queen’s red blood cell dilution | 2 drops of (50 L) tom/kitten’s plasma |
| 7. Negative control: | 2 drops of (50 L) tom/kitten’s red blood cell dilution | 2 drops of (50 L) tom/kitten’s plasma |
| 8. Incubate 30 minutes at C and also at and C. |
| 9. Centrifuge 1 minute. |
| 10. Examine the supernatant for any haemolysis. Any haemolysis indicates also incompatibility. |
| 11. Rotate tubes between the fingers to mix and examine for agglutination. The presence of agglutination indicates a positive test and | tom or kitten/queen incompatibility. |
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