Immunization against Lamb Haemonchosis with a Recombinant Somatic Antigen of Haemonchus contortus (rHcp26/23)

Figure 4

Serum-specific IgG response, determined by ELISA, of lambs along immunization with rHcp26/23 and challenge with 16000 third-stage larvae of H. contortus, against rHcp26/23 (a) and adult soluble extract of the parasite (ASE). G1 (■): lambs ($n=5$) immunized with 3 doses (100 $\mu$g each) of recombinant p26/23 (rHcp26/23) purified under nondenaturing conditions and challenged; G2 (♦): lambs vaccinated with 3 doses of rHcp26/23 purified under denaturing conditions and challenged; G3 (▲): lambs receiving adjuvant injections and challenged; G4 (): unvaccinated and challenged lambs. Arrow: day of experimental challenge. Values are means $\pm$ standard deviation of three determinations.

(a)

(b)