Effect of Sodium Lauryl Sulfate-Fumaric Acid Coupled Addition on the In Vitro Rumen Fermentation with Special Regard to Methanogenesis

Table 2

Effect of treatment systems on gas production and output of ${CO}_2$ and ${CH}_4$ by mixed rumen microorganisms after 24 hours.

Parameter	Control (no additives)	SLS-addition (defaunation)	Fumaric acid addition	SLS-Fumaric acid coupled addition	L.S.D.
Gas volume (mL)	$25.10^{\left(\text{a}\right)}$$\pm$ 0.83	${20.50}^{\left(\text{ab}\right)}$$\pm$ 0.42	${25.20}^{\left(\text{b}\right)}$$\pm$ 1.24	${16.90}^{\left(\text{ab}\right)}$$\pm$ 0.43	2.41
$\text{C}{\text{O}}_2$ ($\mu$moL)	$318.4^{\left(\text{a}\right)}$± 11.70	${249.6}^{\left(\text{ab}\right)}$± 5.88	${301.2}^{\left(\text{b}\right)}$± 17.13	${190.6}^{\left(\text{ab}\right)}$$\pm$ 6.56	33.76
$\text{C}{\text{H}}_4$ ($\mu$moL)	${144.4}^{\left(\text{a}\right)}$$\pm$ 5.36	${118.8}^{\left(\text{ab}\right)}$$\pm$ 3.65	${128.4}^{\left(\text{ac}\right)}$$\pm$ 8.64	${82.2}^{\left(\text{abc}\right)}$$\pm$ 3.09	15.84

Data presented as means $\pm$ SE, $N$$=$ 5
Values having the same letter in the same raw are significantly different at $P<0\mathrm{\hspace{0.17em}\hspace{0.17em}}.05.$