Effect of Sodium Lauryl Sulfate-Fumaric Acid Coupled Addition on the In Vitro Rumen Fermentation with Special Regard to Methanogenesis

Table 4

Effect of treatment systems on extracellular cellulase activity, amount of substrate truly degraded, microbial mass, and biomass yield after 24 hours in vitro incubation.

Parameter	Control (no additives)	SLS-addition (defaunation)	Fumaric acid addition	SLS-Fumaric acid coupled addition	L.S.D.
Extracellular cellulase activity (mmoL of glucose equivalent/min)	4.30 ± 0.11	4.27 ± 0.07	4.29 ± 0.05	4.28 ± 0.07	NS
Amount of substrate truly degraded (mg)	${78.73}^{\left(\text{a}\right)}$ ±1.33	${71.39}^{\left(\text{ab}\right)}$ ± 0.66	${78.94}^{\left(\text{b}\right)}$ ± 1.98	${65.63}^{\left(\text{ab}\right)}$ ± 0.69	3.853
Microbial mass generated (mg)	${23.51}^{\left(\text{a}\right)}$ ± 0.49	${26.29}^{\left(\text{ab}\right)}$ ± 0.26	${23.50}^{\left(\text{b}\right)}$ ± 0.75	28.45 ± 0.26	1.450
Microbial yield/mmoL ATP generated (${\text{Y}}_{\text{ATP}})$	${16.80}^{\left(\text{a}\right)}$ ± 1.14	${22.93}^{\left(\text{ab}\right)}$ ± 0.72	${16.33}^{\left(\text{b}\right)}$ ± 1.52	${30.92}^{\left(\text{ab}\right)}$ ± 1.32	3.632

Data presented as means $\pm$ SE, $N$$=$ 5
Values having the same letter in the same raw are significantly different at $P<\mathrm{\hspace{0.17em}\hspace{0.17em}}0.05.$