Identification of C-Kit-Positive Interstitial Cells in the Dog Lower Urinary Tract and Relationship with Smooth Muscle and Nerves. Hypotheses for a Likely Pacemaker Role.

Table 1

Primary and secondary antibodies used for immunohistochemistry and immunofluorescence, their sources, and working dilution.

Primary antisera tested, working dilution	Source and code	Secondary antisera, working dilution	Secondary antisera, working dilution
Mouse Monoclonal Anti-Human:		biotinylated goat anti mouse IgG, 1 :  25	Vector Lab. Inc., USA, BA-9200
C-kit oncoprotein   (CD117), 1 : 10	Novocastra, UK, NCL-CD117
Smooth muscle actin   (clone 1A4), 1 : 1000	DakoCytomation, Italy, H-7114
Rabbit Policlonal Anti-:		biotynilated goat anti rabbit IgG, 1 : 100	Dakocytomation, Italy, Z0421
Protein Gene Product  9.5 (PGP9.5, Ubiquitin  C-Terminal Hydrolase),  1 : 1000	Chemicon Int. Inc., USA, AB-1761
Neuronal nitric oxide  synthase (NOS1) (R-20),  1 : 200	Santa Cruz Biotechnology, Inc.,USA, Sc-648