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Veterinary Medicine International
Volume 2011, Article ID 162878, 6 pages
http://dx.doi.org/10.4061/2011/162878
Research Article

Fertilization Rate and Number of Embryos on Day 2 after Intrauterine and Deep Intrauterine Insemination Using Frozen-Thawed Boar Semen in Multiparous Sows

Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand

Received 16 April 2010; Accepted 23 July 2010

Academic Editor: Kyle Orwig

Copyright © 2011 Kakanang Buranaamnuay et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present study determines fertilization rate and number of embryos on Day 2 after intrauterine insemination (IUI) and deep intrauterine insemination (DIUI) using frozen-thawed (FT) boar semen in multiparous sows. Twelve crossbred Landrace × Yorkshire multiparous sows were included. The sows were inseminated at 24 h after oestrus detection and reinseminated every 12 h until ovulation took place. The inseminations were conducted using IUI with 2 × 1 0 9  FT sperm per dose ( 𝑛 = 6 ) and DIUI with 1 × 1 0 9  FT sperm per dose ( 𝑛 = 6 ). The sows were slaughtered at 4 5 . 1 ± 7 . 2  h after ovulation. Embryos and unfertilized oocytes were flushed from the oviducts. IUI yielded a better fertilization rate than DIUI (66.0% versus 31.0%, 𝑃 < . 0 0 1 ). The number of embryos was 1 3 . 5 ± 2 . 7 and 6 . 6 ± 3 . 2 embryos/sow in IUI and DIUI groups, respectively ( 𝑃 = . 0 8 ). The proportion of sows having unilateral fertilization was higher in the DIUI (3/5) than the IUI group (1/6). In conclusion, IUI with at least 2 × 1 0 9 total number of FT boar spermatozoa is recommended.