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Veterinary Medicine International
Volume 2011, Article ID 267102, 5 pages
http://dx.doi.org/10.4061/2011/267102
Research Article

Detection of Mycobacterium avium subsp. paratuberculosis by a Direct In Situ PCR Method

1Instituto de Patobiología, CICVyA, INTA, De Los Reseros y Dr. Nicolás Repetto S/N, Hurlingham, 1686 Buenos Aires, Argentina
2Experimental Pathology Section, Department of Pathology, Instituto Nacional de la Nutrición “Salvador Zubirán”, Vasco de Quiroga 15, Colonia Seccion XVI, Tlalpan,14000 Mexico City, DF, Mexico
3Instituto de Biotecnología, CICVyA, INTA, De Los Reseros y Dr. Nicolás Repetto S/N, Hurlingham, 1686 Buenos Aires, Argentina

Received 12 January 2011; Revised 3 March 2011; Accepted 26 April 2011

Academic Editor: Jesse M. Hostetter

Copyright © 2011 Fernando Delgado et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. G. W. Dunkin, “Paratuberculosis of cattle and sheep,” Proceedings of the Royal Society of Medicine, vol. 30, no. 2, pp. 83–90, 1936. View at Google Scholar
  2. L. Sechi, M. Mura, F. Tanda et al., “Identification of Mycobacterium avium subsp. paratuberculosis in biopsy specimens from patients with Crohn's disease identified by in situ hybridization,” Journal of Clinical Microbiology, vol. 39, no. 12, pp. 4514–4517, 2001. View at Publisher · View at Google Scholar
  3. F. A. K. El-Zaatari, M. S. Osato, and D. Y. Graham, “Etiology of Crohn's disease: the role of Mycobacterium avium paratuberculosis,” Trends in Molecular Medicine, vol. 7, no. 6, pp. 247–252, 2001. View at Publisher · View at Google Scholar · View at Scopus
  4. D. J. Brees, S. B. Reimer, N. F. Cheville, A. Florance, and C. O. Thoen, “Immunohistochemical detection of Mycobacterium paratuberculosis in formalin-fixed, paraffin-embedded bovine tissue sections,” Journal of Veterinary Diagnostic Investigation, vol. 12, no. 1, pp. 60–63, 2000. View at Google Scholar · View at Scopus
  5. F. Delgado, D. Etchechoury, A. Gioffré et al., “Comparison between two in situ methods for Mycobacterium avium subsp. paratuberculosis detection in tissue samples from infected cattle,” Veterinary Microbiology, vol. 134, no. 3-4, pp. 383–387, 2009. View at Publisher · View at Google Scholar · View at Scopus
  6. S. A. Martinson, P. E. Hanna, B. O. Ikede et al., “Comparison of bacterial culture, histopathology, and immunohistochemistry for the diagnosis of Johne's disease in culled dairy cows,” Journal of Veterinary Diagnostic Investigation, vol. 20, no. 1, pp. 51–57, 2008. View at Google Scholar · View at Scopus
  7. M. Jeyanathan, D. C. Alexander, C. Y. Turenne, C. Girard, and M. A. Behr, “Evaluation of in situ methods used to detect Mycobactenum avium subsp. paratuberculosis in samples from patients with Crohn's disease,” Journal of Clinical Microbiology, vol. 44, no. 8, pp. 2942–2950, 2006. View at Publisher · View at Google Scholar · View at Scopus
  8. E. Sanna, C. J. Woodall, N. J. Watt et al., “In situ-PCR for the detection of Mycobacterium paratuberculosis DNA in paraffin-embedded tissues,” European Journal of Histochemistry, vol. 44, no. 2, pp. 179–184, 2000. View at Google Scholar · View at Scopus
  9. R. Hernández-Pando, M. Jeyanathan, G. Mengistu et al., “Persistence of DNA from Mycobacterium tuberculosis in superficially normal lung tissue during latent infection,” Lancet, vol. 356, no. 9248, pp. 2133–2138, 2000. View at Publisher · View at Google Scholar · View at Scopus
  10. C. E. Greer, S. L. Peterson, N. B. Kiviat, and M. M. Manos, “PCR amplification from paraffin-embedded tissues. Effects of fixative and fixation time,” American Journal of Clinical Pathology, vol. 95, no. 2, pp. 117–124, 1991. View at Google Scholar · View at Scopus
  11. M. M. Arnold, S. Srivastava, J. Fredenburgh, C. R. Stockard, R. B. Myers, and W. E. Grizzle, “Effects of fixation and tissue processing on immunohistochemical demonstration of specific antigens,” Biotechnic and Histochemistry, vol. 71, no. 5, pp. 224–230, 1996. View at Google Scholar · View at Scopus
  12. D. Cousins, R. Whittington, I. Marsh, A. Masters, R. J. Evans, and P. Kluver, “Mycobacteria distinct from Mycobacterium avium subsp. paratuberculosis isolated from the faeces of ruminants possess IS900-like sequences detectable by IS900 polymerase chain reaction: implications for diagnosis,” Molecular and Cellular Probes, vol. 13, no. 6, pp. 431–442, 1999. View at Publisher · View at Google Scholar · View at Scopus
  13. A. S. Motiwala, M. Strother, A. Amonsin et al., “Molecular epidemiology of Mycobacterium avium subsp. paratuberculosis: evidence for limited strain diversity, strain sharing, and identification of unique targets for diagnosis,” Journal of Clinical Microbiology, vol. 41, no. 5, pp. 2015–2026, 2003. View at Publisher · View at Google Scholar · View at Scopus
  14. P. Kralik, I. Slana, A. Kralova, V. Babak, R. H. Whitlock, and I. Pavlik, “Development of a predictive model for detection of Mycobacterium avium subsp. paratuberculosis in faeces by quantitative real time PCR,” Veterinary Microbiology, vol. 149, no. 1-2, pp. 133–138, 2011. View at Publisher · View at Google Scholar