The First Report of Mycobacterium celatum Isolation from Domestic Pig (Sus scrofa domestica) and Roe Deer (Capreolus capreolus) and an Overview of Human Infections in Slovenia

Table 1

Details on diagnostics of M. celatum isolates from animals.

Animal	Year	Specimen	Visible lesions	Smear microscopy^2	Growth on media	Growth time (days)	PCR	GenoType^7	16S rDNA sequencing
Pig	2000	Submandibular	Yes	Neg^3	LJ-G^4, S^5, MGIT^6	19 (MGIT)	IS1245 Neg	M. celatum	M. celatum ^8
		lymph nodes^1				28 (LJ-G)	FR300 Neg
						34 (S)
Roe deer	2010	Lymph node	No	Neg	LJ-G, S, MGIT	34 (LJ-G, S, MGIT)	IS1245 Neg	M. celatum	M. celatum
									type 1 and 3
		Liver	No	Neg	Neg	—	Neg	—	—

¹Pooled specimen from 30 animals.
²Ziehl-Neelsen staining.
³Negative.
⁴Löwenstein-Jensen medium supplemented with glycerol.
⁵Stonebrink medium.
⁶Mycobacteria Growth Indicator Tube (Becton Dickinson, USA), liquid medium.
⁷GenoType Mycobacterium CM and AS culture identification kits (Hain Lifescience, Germany).
⁸Identification provided by the National Reference Center for Mycobacteria, Research Center Borstel, Germany.