Veterinary Medicine International / 2011 / Article / Tab 1

Research Article

Intensity of Oestrus Signalling Is the Most Relevant Indicator for Animal Well-Being in High-Producing Dairy Cows

Table 1

Intra- and interassay measurements of relative P4 concentrations [low (levels 1-2, <3.0 nM/L), medium (levels 3-4, 3.1–7.5 nM/L) or high (levels 5-6, >7.6 nM/L)), studied in fresh serum, plasma, or milk, or frozen-thawed serum or plasma samples, comparing different sample volumes, at various days (day 0, 7, or 20) of AI, provided by the eProCheck800. Number of observations per sample and coefficient of variation (CV) are indicated.

IntraassayInterassay
Sample statusFreshFrozen-thawedFreshFrozen-thawed

Day sampledd0d20d0d7d0d20d0d0d0

Sample typeSerumPlasmaMilkMilkSerumPlasmaSerumPlasmaSerumSerumplasma

Sample size, μL1515302015151515151515

No. of replicates5101077777767

ReplicatesP4-Levels
1lowmedlowhighmedhighhighhighlowlowhigh
2lowmedmedhighhighhighhighhighlowlowhigh
3lowmedlowhighmedhighhighhighlowlowhigh
4lowhighmedhighhighhighhighhighmedlowhigh
5lowmedlowhighmedhighhighhighlowlowhigh
6medmedhighmedhighhighhighlowlowhigh
7medmedhighhighmedhighhighlowlowhigh
8medlowhigh
9medmed
10medlow

CV (%)<5.015.135.1<5.022.013.2<5.0<5.033.1<5.0<5.0

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