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Veterinary Medicine International
Volume 2011 (2011), Article ID 642931, 5 pages
http://dx.doi.org/10.4061/2011/642931
Research Article

Ram Sperm Motility Parameters under The Influence of Epidermal Growth Factor

1Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture Nitra, Trieda A. Hlinku 2, 949 76 Nitra, Slovakia
2Institute for Farm Animal Genetics and Reproduction, Animal Production Research Centre Nitra, Hlohovecká 2, 95141 Nitra, Slovakia

Received 2 December 2010; Accepted 2 March 2011

Academic Editor: Cynthia L. Baldwin

Copyright © 2011 Eliška Špaleková et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Epidermal growth factor (EGF) is one of the important cytokines that play a role in fertility. It is known that EGF affects both male and female reproduction, but its effect on sperm parameters is not fully understood. Up to the present, the effect of EGF on ram sperm motility parameters has not been published. We analyzed motility parameters of ejaculates after 24, 48, and 72 hours from the EGF addition. EGF was added to chilled ram sperm at concentrations of 0, 100, 200, and 400 ng⋅ml−1. Analyses were realized using computer, assisted semen analyzer (CASA)—Hamilton Thorn motility analyzer (version 7). The effect of EGF was already visible after 30 min of incubation. Significant effect on ram sperm total motility and progressive movement was observed at higher EGF concentrations after 48 h of incubation. Our results show that EGF affects sperm motility parameters depending on concentration and time of exposure.