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Veterinary Medicine International
Volume 2011 (2011), Article ID 686570, 7 pages
http://dx.doi.org/10.4061/2011/686570
Research Article

The Number of Grafted Fragments Affects the Outcome of Testis Tissue Xenografting from Piglets into Recipient Mice

Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, SK, Canada S7N 5B4

Received 3 May 2010; Accepted 31 May 2010

Academic Editor: Stefan Schlatt

Copyright Β© 2011 Sepideh Abbasi and Ali Honaramooz. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

To optimize the procedure for testis tissue xenografting, we grafted 2, 4, 8, or 16 small fragments of immature porcine testis tissue under the back skin of immunodeficient castrated mice ( 𝑛 = 1 0 mice/group). At 8 months post grafting, the graft recovery rate did not differ between groups; however, not only the total but also the average graft weights were higher (by ∼ 12-fold and ∼ 2.5-fold, resp.) in mice receiving 16 fragments than those receiving 2 fragments ( 𝑃 < . 0 5 ). The recipient mice with 16 fragments had the largest vesicular glands (indicators of testosterone release by the grafts) compared with those with 2 fragments ( 𝑃 = . 0 0 7 ). The grafts in the group of 16 fragments also had more ( 𝑃 < . 0 5 ) percentage of tubules with round spermatids than those of the group of mice receiving 2 fragments. Therefore, recipient mice can be grafted with at least 16 testis tissue fragments for optimal results.