Protein C Activity in Dogs: Adaptation of a Commercial Human Colorimetric Assay and Evaluation of Effects of Storage Time and Temperature

Table 1

Plasma storage conditions for Phase 1 (a) and Phase 2 (b).

(a)

Sample	Storage conditions
Control	NA (fresh plasma)
Aliquot 1.1	4 d (7 dogs) or 7 d (2 dogs) at −80°C
Aliquot 1.2	4 d (7 dogs) or 7 d (2 dogs) at −80°C, then thawed, refrozen at −20°C, and shipped with a cold pack the next day overnight to an independent laboratory^A; assay performed 2 d after initial thaw from −80°C
Aliquot 1.3	4 d (7 dogs) or 7 d (2 dogs) at −80°C, then thawed, refrozen at −20°C, and packaged the next day identically to Aliquot 1.2 (package kept at room temperature); assay performed 2 d after initial thaw from −80°C
Aliquot 1.4	4 d (7 dogs) or 7 d (2 dogs) at −80°C, then thawed and refrozen at −80°C for another 4 d
Aliquot 1.5	4 d (7 dogs) or 7 d (2 dogs) at −80°C, then thawed and refrozen at −80°C for another 7 d

^ACornell University, Animal Health Diagnostic Center.

(b)

Sample	Storage conditions
Control	NA (fresh plasma)
Aliquot 2.1	7 d at −80°C
Aliquot 2.2	7 d at −20°C
Aliquot 2.3	28 d at −80°C
Aliquot 2.4	28 d at −20°C