Protein C Activity in Dogs: Adaptation of a Commercial Human Colorimetric Assay and Evaluation of Effects of Storage Time and Temperature

Table 2

Comparison of standard curves generated 1 week apart, using pooled canine plasma (Curves 1 and 2), and a curve generated using the manufacturer’s standard protocol (Mfr Std). Results for protein C (PC) activity shown here are hypothetical, calculated by inserting arbitrary optical density (OD) values into the regression equations for the curves (Curve 1: c = [1278.276 × d] − 12.397; Curve 2: c = [1257.324 × d] − 9.60; Mfr Std curve: c = [515.734 × d] − 0.608; where c = PC activity and d = OD). Actual instrument output for Curve 2 is shown in Figure 1.

	Protein C activity (%)
OD	Curve 1	Curve 2	Mfr Std curve
0.02	13	16	10
0.04	39	41	20
0.06	64	66	30
0.08	90	91	41
0.10	115	116	51
0.12	141	141	61