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Veterinary Medicine International
Volume 2011 (2011), Article ID 953985, 8 pages
http://dx.doi.org/10.4061/2011/953985
Research Article

Bovine Tuberculosis in a Nebraska Herd of Farmed Elk and Fallow Deer: A Failure of the Tuberculin Skin Test and Opportunities for Serodiagnosis

1National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture (USDA), Ames, IA 50010, USA
2National Veterinary Services Laboratories (NVSL), Animal and Plant Health Inspection Service (APHIS), USDA, Lincoln, NE 68516, USA
3NVSL, APHIS, USDA, Fort Collins, CO 80526-8117, USA
4NVSL, APHIS, USDA, Ames, IA 50010, USA
5North American Deer Farmers Association, Lake City, MN 55041, USA
6Chembio Diagnostic Systems, Inc., Medford, NY 11763, USA

Received 10 January 2011; Accepted 20 February 2011

Academic Editor: Michael D. Welsh

Copyright © 2011 W. Ray Waters et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In 2009, Mycobacterium bovis infection was detected in a herd of 60 elk (Cervus elaphus) and 50 fallow deer (Dama dama) in Nebraska, USA. Upon depopulation of the herd, the prevalence of bovine tuberculosis (TB) was estimated at ∼71–75%, based upon histopathology and culture results. Particularly with elk, gross lesions were often severe and extensive. One year ago, the majority of the elk had been tested for TB by single cervical test (SCT), and all were negative. After initial detection of a tuberculous elk in this herd, 42 of the 59 elk were tested by SCT. Of the 42 SCT-tested elk, 28 were TB-infected with only 3/28 reacting upon SCT. After SCT, serum samples were collected from the infected elk and fallow deer from this herd at necropsy and tested by three antibody detection methods including multiantigen print immunoassay, cervidTB STAT-PAK, and dual path platform VetTB (DPP). Serologic test sensitivity ranged from 79 to 97% depending on the test format and host species. Together, these findings demonstrate the opportunities for use of serodiagnosis in the rapid detection of TB in elk and fallow deer.