Analysis of In Vitro Effects of Sex Steroids on Lymphocyte Responsiveness in Murrah Buffaloes (Bubalus bubalis)

Table 1

Dose-dependent effect of estradiol 17β on lymphocyte proliferation in vitro in Murrah buffaloes of different sex and age.

Age group	Sex	Estradiol 17β concentration (pg/mL)				Sex* group mean	Group mean	Sex mean
		10	100	500	1000
Group-I	Male	0.98±0.03	1.04±0.04	0.93±0.04	0.87±0.04	0.95±0.02	0.95^B ± 0.01	0.97±0.01 (Male)
	Female	0.93±0.03	0.99±0.02	0.93±0.02	0.93±0.02	0.94±0.01
Group-II	Male	0.95±0.04	1.01±0.05	0.97±0.04	0.91±0.01	0.96±0.02	0.95^B ± 0.01
	Female	0.93±0.04	1.01±0.03	0.96±0.02	0.83±0.02	0.93±0.02		0.99±0.01 (Female)
Group-III	Male	0.91±0.02	1.12±0.06	1.07±0.05	0.86±0.05	0.99±0.03	1.04^A ± 0.01
	Female	0.99±0.02	1.11±0.08	1.14±0.08	1.14±0.08	1.09±0.03
Treatment mean		0.95^b ± 0.01	1.05^a ± 0.02	1.00^a± 0.02	0.93^b ± 0.02

Values with superscript a, b within same row and A, B within same column differ significantly (𝑃<0.05). CD at 𝑡 5%: sex: 0.03, group: 0.04, treatment: 0.05, sex* group: 0.06 and sex*group*treatment: 0.129.