Analysis of In Vitro Effects of Sex Steroids on Lymphocyte Responsiveness in Murrah Buffaloes (Bubalus bubalis)

Table 2

Dose dependent effect of progesterone on lymphocyte proliferation in vitro in Murrah buffaloes of different sex and age.

Age group	Sex	Progesterone concentration (ng/mL)				Sex* group mean	Group mean	Sex mean
		10	100	500	1000
Group-I	Male	0.98±0.03	0.90±0.03	0.92±0.02	0.86±0.01	0.91±0.01	0.92±0.01	0.90±0.01 (Male)
	Female	0.99±0.07	0.96 ±0.04	0.93±0.02	0.83±0.04	0.92±0.02
Group-II	Male	1.09±0.07	1.07 ±0.05	0.87±0.03	0.73±0.02	0.94±0.03	0.94±0.01
	Female	1.01±0.01	1.00±0.04	0.89±0.02	0.76±0.01	0.94±0.02		0.97±0.01 (Female)
Group-III	Male	1.06±0.05	0.89±0.04	0.77±0.04	0.71±0.05	0.86±0.03	0.96±0.01
	Female	1.14±0.07	1.07±0.05	1.05±0.05	0.99±0.03	1.06±0.02
Treatment mean		1.06^a ± 0.02	0.98^b ± 0.02	0.90^c ± 0.02	0.81^d ± 0.02

Values with different superscript within same row differ significantly (𝑃<0.05). CD at 𝑡 5%: sex: 0.03, treatment: 0.05, sex* group: 0.06 and sex* group* treatment: 0.123.