Veterinary Medicine International

Research Article

Differential Gene Expression Segregates Cattle Confirmed Positive for Bovine Tuberculosis from Antemortem Tuberculosis Test-False Positive Cattle Originating from Herds Free of Bovine Tuberculosis

Table 1

Genes selected for qPCR analysis, the nucleic acid sequence and concentration (nM) of PCR primers for those genes (forward primer (F) and reverse primer (R)), the PCR efficiency (E), and the PCR amplicon sizes (bp).


	Gene symbol	Gene name	Primer (5′-3′)	Primer conc. (nM)	PCR eff. (E)	Amplicon size (bp)

#	ARF3	ADP-ribosylation factor 3	F: TTGCCTAATGCCATGAATGC R: CACAGGTGGCCTGAATGTA	300	1.817	91
#	BOLA-DMA	Major histocompatibility complex, class II, DM α-chain	F: TTGTTGGCTTGGTCCTCTTC R: ACACCTCCTGCTTGGATGG	300	1.975	105
#	BOTL08_C07	Unknown	F: ATCACTTCCCGCCTCCTTAG R: AGGCAGGTGACCAAGGAAAC	600	1.925	92
#	CXCL2	C-X-C ligand 2 (GRO-alpha)	F: AACAAGGCTAGTGCCAACTG R: CCACTGAGGCTGCTGGAG	300	1.912	68
#	DDX5	DEAD (Asp-Glu-Ala-Asp) box polypeptide 5	F: AGAGATCTGGTGGGTAGCTTTA R: ACCCTATCCTCTCCTTGCAAAC	300	1.917	79
#	IL-4	Interleukin-4	F: GCCACACGTGCTTGAACAAA R: TGCTTGCCAAGCTGTTGAGA	450	1.910	63
#	LTBR	Lymphotoxin beta receptor (TNFR superfamily member3)	F: CCGGAGTGACGAGGAAGAC R: CAAAACTCGCCCTTATACCTTG	450	1.859	104
#	PPP2R5B	Protein phosphatase 2, regulatory subunit , beta isoform	F: GTGGTCCTGGCAACAGAAC R: CTGGAGCCCAGCTTTGTG	300	1.895	110
#	PRKCI	Protein kinase C, iota	F: CAAGGACCCAAAGGAACGATT R: ACCACCTGCTTTTGCTCCAT	300	1.897	114
#	PTGS2	Prostaglandin-endoperoxide synthase 2(cyclooxygenase-2)	F: CGACACCAAGAACGTATTCCTA R: GAGATGTGGAAAAGAAGCATTG	300	1.930	105
*	BOLA-DRA	MHC class II DR alpha	F: GCTCTGGTGGGCATCATTG R: CCTCGGCGTTCAACGGTG	300	1.910	77
*	TPRA1	Transmembrane protein, adipocyte associated 1	F: GTGCGCAGACATCATTGAG R: GGCGCAAAGAAGCTGAAG	450	1.974	72
*	TRIM13	Tripartite motif-containing 13	F: CTGGCACGTTCATTAGCAAG R: GGCCAAGCAGAATGACCAC	300	1.962	69
*	FCGRT	Fc fragment of IgG, receptor, transporter, alpha	F: GGCCCGAATCGTTGTGTT R: GAAGCCCAAGGCTTACACC	450	1.822	81
*	TMX4	Thioredoxin-related transmembrane protein 4	F: ACCTTGACTTGTGCTCACTT R: TGGAGGTACCACTGGAACTG	300	1.993	85
*	BOTL11_A05	Unknown	F: CACACTCTATGGCGCAAATC R: CCCTGGACCACCACCTCTA	300	1.903	75
*	RPL19	Ribosomal protein L19	F: GGCTCCAGGCCAAGAAAG R: AATTGCCGAGGCCACTATG	300	1.972	106
§	CSF3	Colony stimulating factor 3 (granulocyte)	F: CTGGGTGAGACTGGGAAATG R: TCTCTCACACCCCGTCACA	300	1.959	62
§	GCP2	Granulocyte chemotactic protein 2 (CXCL6)	F: CATTGGAATGCTGTATATGGAGAT R: TCTTCCAAAGGTCAAGAGTAAGA	300	1.874	122
§	IL-10	Interleukin-10	F: CTTGTCGGAAATGATCCAGTTTT R: TCAGGC CCGTGG TTCTCA	300	1.948	66
§	IL-10RA	Interleukin-10 receptor A	F: GTCACCCTGCCACTGATCAC R: GGCAGCGTGCAGCTGAAATC	300	1.828	84
§	IL-6	Interleukin-6	F: GGCTCCCATGATTGTGGTAGTT R: GCCCAGTGGACAGGTTTCTG	300	1.873	64
§	IL-12p40	Interleukin-12, p40 subunit	F: CAAACCAGACCCACCCAAGA R: GACCTCCACCTGCCGAGAA	300	1.896	64
§	IL-15	Interleukin-15	F: GGCTGGCATTCATGTCTTCA R: CATACT GCCAGT TTGCTTCTGTTT	300	1.850	74
§	IL-18	Interleukin-18	F: GAAAATGATGAAGACCTGGAATCA R: ACTTGGTCATTCAAATTTCGTATGA	300	1.896	84
§	IL-1b	Interleukin-1 beta	F: AAGCAGGCGCATCTGTGAA R: ATGGCACTCTAACCCGGAAA	450	1.915	70
§	IL1R2	Interleukin-1 receptor 2	F: ATACCTGTGCCATGACGTATGC R: CGGAGTTTGATATTCCTGGTGAT	300	1.923	67
§	IL2	Interleukin-2	F: TGATGCAACAGTAAACGCTGTAG R: GAGAGGCACTTAGTGATCAAGTC	450	1.928	95
§	IL-1α	Interleukin-1 alpha	F: TTGGTGCACATGGCAAGTG R: GCACAGTCAAGGCTATTTTTCCA	450	1.948	72
§	IL-8	Interleukin-8	F: GGAAAAGTGGGTGCAGAAGGT R: GGTGGTTTTTTCTTTTTCATGGA	100	1.888	80
§	INF-γ	Interferon, gamma	F: TGGCATGTCAGACAGCACTTG R: CCTGAAGCGCCAGGTATAAGG	450	1.932	96
§	TGFβ	Transforming growth factor, beta	F: CTGAGCCAGAGGCGGACTAC R: TGCCGTATTCCACCATTAGCA	300	1.897	63
§	TNFα	Tumor necrosis factor, alpha	F: TCTACCAGGGAGGAGTCTTCCA R: GTCCGGCAGGTTGATCTCA	300	1.871	68
	SDHA	Succinate dehydrogenase complex subunit A	F: CCACGCCAGGGAGGACTTC R: CGTAGGAGAGCGTGTGCTTC	300	1.879	116

^#Genes that showed substantial altered expression within a group of cattle in microarray studies.
*Genes that had differential power between groups of cattle using microarray expression data analyzed with MAANOVA.
^§Genes that were selected from the literature as being relevant to the bTB infection.