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Veterinary Medicine International
Volume 2012, Article ID 394189, 7 pages
Research Article

Evaluation of Protein Concentration and Limiting Amino Acids Including Lysine and Met + Cys in Prestarter Diet on Performance of Broilers

1Department of Animal and Poultry, Health and Nutrition, Faculty of Veterinary Medicine, University of Tehran, P.O. Box 14155-6453, Tehran, Iran
2Faculty of Veterinary Medicine, University of Tehran, P.O. Box 14155-6453, Tehran, Iran

Received 18 May 2012; Revised 29 August 2012; Accepted 3 September 2012

Academic Editor: Pedro J. Ginel

Copyright © 2012 Mohsen Farkhoy et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Four experiments were conducted, in two stages, to evaluate protein and limiting amino acids' (lysine and methionine + cystine) levels in pre-starter diets on broilers’ performance. In each experiment of Stage 1, 640 new-born male Ross 308 cockerels were randomly allocated to eight dietary treatments with a 2 × 4 factorial arrangement. In experiment 1-1, two levels of crude protein (CP: 21% and 23.2%) and four levels of Lys (1.2, 1.3, 1.4, and 1.5%) and in experiment 1-2, two levels of CP (21 and 23.2%) and four levels of Met + Cys (0.85, 0.90, 0.95, and 1.00%) were used. In Stage 2, the optimum levels of Lys and Met + Cys obtained from Stage 1 (1.3 and 1.5% Lys, 0.90 and 1.00% Met + Cys in experiment 1-1 and 1-2, resp.) with two levels of CP (21 and 23.2%) were used in two separate simultaneous experiments with a 2 × 2 factorial arrangement for male and female birds. The levels of CP significantly influenced BWG and FCR in experiment 1-1. Dietary levels of Lys affect BWG (experiment 1-1) and FI (experiments 1-1 and 2-1) significantly. In experiments 1-2 and 2-2, the different levels of Met + Cys did not affect BWG, FI, and FCR of male or female broilers. The results of these experiments indicated that the optimal level of dietary protein and Lys were 23.2% and 1.5%, respectively. Diets with 1% Met + Cys caused optimal performance.