T-Cell and Antibody Responses to Mycobacterial Antigens in Tuberculin Skin-Test-Positive Bos indicus and Bos taurus Cattle in Ethiopia

Figure 2

Proportion of IFN-𝛾 producing T-lymphocytes in Holstein and zebu cattle. Enzyme immunospot assay (ELispot) was used for the assessment of IFN-𝛾 producing T cell in response to novel mycobacterial antigens. Responder frequencies for Holstein and zebu cows were calculated using a cutoff for positivity >25 SFC/million). Although both breeds recognized the majority antigens studied at comparable frequencies, antigens such as CFP-10, ESAT-6, Rv0287, Rv0288, MPB87, Acr-2, Rv3616c, and Rv3879c were recognized at higher frequencies in Zebu than in Holstein.