Table of Contents Author Guidelines Submit a Manuscript
Veterinary Medicine International
Volume 2014 (2014), Article ID 187842, 7 pages
http://dx.doi.org/10.1155/2014/187842
Research Article

Characterization of Mycobacterium bovis from Humans and Cattle in Namwala District, Zambia

1Institute of Economic and Social Research, University of Zambia, P.O. Box 30900, Lusaka, Zambia
2Norwegian Veterinary Institute, P.O. Box 750, 0106 Oslo, Norway
3Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka, Zambia
4Tuberculosis Laboratory, Department of Microbiology and Pathology, University Teaching Hospital, P/Bag RW1X, Lusaka, Zambia
5The Roslin Institute, College of Medicine and Veterinary Medicine, University of Edinburgh, Easter Bush, Roslin, Midlothian EH25 9RG, UK
6Department of Arctic and Marine Biology, University of Tromsø-The Arctic University of Norway, Stakkevollveien 23, 9010 Tromsø, Norway

Received 19 August 2013; Accepted 18 March 2014; Published 14 April 2014

Academic Editor: Timm C. Harder

Copyright © 2014 Sydney Malama et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Tuberculosis remains a major public health problem in Zambia. While human to human transmission of Mycobacterium tuberculosis is of major importance in driving the tuberculosis epidemic, the impact of Mycobacterium bovis transmission from infected cattle is largely unknown. This cross-sectional study aimed at molecular characterization of M. bovis in humans and cattle. A total of 100 human sputum samples and 67 bovine tissues were collected and analyzed for the presence of mycobacteria. Of 65 human samples that harbored acid fast bacteria (AFB), 55 isolates were obtained of which 34 were identified as M. tuberculosis and 2 as M. bovis. AFB-positive bovine samples () yielded 47 mycobacterial isolates among which 25 were identified as M. bovis and no M. tuberculosis was found. Among the M. bovis isolates, spoligotyping revealed a high homogeneity in genotypes circulating in Namwala district. Human and cattle isolates shared identical MIRU-VNTR genotypes, suggesting that transmission between the two hosts may occur. Therefore, this study has documented zoonotic TB in human patients in Namwala district of Zambia. However, further molecular epidemiological studies in the study area are recommended.