Morphometric Evaluation of Interrenal Gland and Kidney Macrophages Aggregates in Normal Healthy Rainbow Trout (Oncorhynchus mykiss) and after Bacterial Challenge with Yersinia ruckeri

Table 1

Oncorhynchus mykiss. Percentage (mean values) of tissue occupied by macrophages aggregates (MA) and MA number/mm² in the interrenal gland and posterior kidney from six groups of Yersinia ruckeri infected rainbow trout (A, B, C D, E, and F) and a control group (Ctrl).

			Interrenal gland										Posterior kidney
Groups	MA Area fraction (%)		Levels				Number of MA/mm^2		Levels				MA Area fraction (%)		Levels					Number of MA/mm^2		Levels
	Mean	Std Error					Mean	Std Error					Mean	Std Error						Mean	Std Error
A	0.79	0.4542			c		420.19	9.757				d	1.10	0.1432				d		440.86	10.335						f
B	0.84	0.4646			c		485.08	9.988			c	d	1.15	0.1482				d		518.44	10.697					e
C	0.98	0.4625			c		708.50	9.940		b			1.41	0.1521			c			749.83	10.976				d
D	1.51	0.5257		b			520.19	11.298			c		3.54	0.1691		b				856.22	12.208			c	d
E	2.35	0.4653	a				777.72	9.994	a				3.44	0.1547		b				944.56	11.167		b	c
F	1.76	0.5236		b			560.03	11.250		b	c		4.80	0.1581	a					1154.88	11.413		b
Ctrl	2.49	0.6056				d	882.41	13.018	a				6.47	0.1901					e	1551.55	13.721	a

Letters represent significant differences (𝑃<0.05) among groups of Yersinia ruckeri-infected fish (A, B, C, D, E, and F) and a control group (Ctrl) based on Tukey analysis run separately for the interrenal gland and the posterior kidney data. Levels not connected by the same letter are significantly different.