Figure 1: Comparison of the mean absorbance values of lysate antigens prepared from dog (ERC-2, T-58) and human (B5931, B5896) isolates using three different substrates (Ultra TMB, SureBlue, and SureBlue Reserve) for antibody detection in the 48 serum specimens from dogs with blastomycosis (24 for each trial: Trial 1 and Trial 2).