Evaluation of Sample Handling Effects on Serum Vitamin E and Cholesterol Concentrations in Alpacas

Table 2

Least squares means serum cholesterol concentrations (µg/dL) in 2 adult male alpacas after exposure of whole blood to the following conditions: exposure to fluorescent room light (light exposure: light or dark), contact with the rubber tube stopper (position: upright or stopper contact), storage duration before serum separation (time: 1, 4, or 24 hours), and temperature during storage (temp.: 4°C or 25°C). The effect of each alpaca was also analyzed. 𝑃 values are reported for the ANOVA 𝐹 statistic of the entire variable.

Variable	Mean (SE)	𝑃 value
Alpaca
A	60.7 (0.21)	<0.0001
B	42.9 (0.21)
Light exposure
Light	51.8 (0.28)	0.99
Dark	51.8 (0.17)
Position
Upright	51.7 (0.21)	0.54
Stopper contact	51.9 (0.21)
Time
1 hr	51.0 (0.25)	0.0004
4 hr	52.1 (0.25)
24 hr	52.3 (0.25)
Temp.
4°C	52.1 (0.28)	0.08
25°C	51.5 (0.16)