Table of Contents Author Guidelines Submit a Manuscript
Veterinary Medicine International
Volume 2014 (2014), Article ID 892421, 6 pages
http://dx.doi.org/10.1155/2014/892421
Research Article

Electrophoretic Analysis of Indian Isolates of Mycoplasma agalactiae and Mycoplasma bovis by SDS-PAGE and Immunoblotting

1Department of Microbiology, Uttar Pradesh Pandit Deen Dayal Upadhayay Pashu Chikitsa Vigyan Vishwa Vidyalaya Evam Go-Anusandhan Sansthan (DUVASU), Mathura 281001, India
2National Referral Laboratory on Mycoplasma, Division of Bacteriology & Mycology, Indian Veterinary Research Institute, Izzatnagar 243122, India
3Division of Bacteriology & Mycology, Indian Veterinary Research Institute, Izzatnagar 243122, India
4Divisionof Animal Science, Central Agricultural Research Institute, Port Blair, A & N Islands 744101, India

Received 27 December 2013; Revised 1 March 2014; Accepted 17 March 2014; Published 3 April 2014

Academic Editor: Praveen Malik

Copyright © 2014 Amit Kumar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. S. Razin, D. Yogev, and Y. Naot, “Molecular biology and pathogenicity of mycoplasmas,” Microbiology and Molecular Biology Reviews, vol. 62, no. 4, pp. 1094–1156, 1998. View at Google Scholar · View at Scopus
  2. A. Kumar, A. K. Verma, and A. Rahal, “Mycoplasma bovis, a multi disease producing pathogen: an overview,” Asian Journal of Animal and Veterinary Advances, vol. 6, no. 6, pp. 537–546, 2011. View at Publisher · View at Google Scholar · View at Scopus
  3. A. Kumar, A. K. Verma, N. K. Gangwar, and A. Rahal, “Isolation, characterization and antibiogram of Mycoplasma bovis in sheep Pneumonia,” Asian Journal of Animal and Veterinary Advances, vol. 7, no. 2, pp. 149–157, 2012. View at Publisher · View at Google Scholar · View at Scopus
  4. M. Lambert, “Contagious agalactia of sheep and goats,” Revue Scientifique et Technique. Office International des Epizooties, vol. 6, pp. 699–711, 1987. View at Google Scholar
  5. A. Kumar, N. C. Srivastava, and V. P. Singh, “Rapid identification of M. agalactiae and M. bovis by immuno binding assay,” Indian Journal of Comparative Microbiology Immunology and Infectious Diseases, vol. 23, no. 2, pp. 161–163, 2002. View at Google Scholar
  6. F. Garrido, J. L. Leon, L. Cuellar, and M. A. Diaz, Contagious Agalactia and Other Mycoplasmal Diseases of Ruminants, Edited by: M. Lambert and G. Jones, Luxembourg, Commission of the European Community, 1987.
  7. R. Nicholas, Contagious Agalactia: An Update. in Cost 826, Mycoplasmas of Ruminants: Pathogenicity, Diagnostics, Epidemiology and Molecular Genetics, Edited by: J. Frey and K. Sarris, 1996.
  8. K. J. MacOwan, T. F. Brand, N. McGillveray, and A. R. Hunter, “Experimental infection of castrated lambs with Mycoplasma agalactiae,” Journal of Hygiene, vol. 93, no. 3, pp. 455–463, 1984. View at Google Scholar · View at Scopus
  9. K. Lagakis, “An economic appraisal of the acute form of contagious agalactia in the transhumant sheep and goats in Greece,” in Cost 826, Mycoplasmas of Ruminants: Pathogenicity, Diagnostics, Epidemiology and Molecular Genetics, J. Frey and K. Sarris, Eds., pp. 100–103, 1996. View at Google Scholar
  10. N. C. Srivastava, Studies on the mycoplasma and acholeplasma of respiratory tract of buffaloes [Ph.D. thesis], Rohilkhand University, Bareilly, India, 1982.
  11. G. Askaa and H. Erno, “Elevation of Mycoplasma agalactiae subsp. bovis to species rank: Mycoplasma bovis (Hale et al). comb. nov,” International Journal of Systematic Bacteriology, vol. 26, no. 3, pp. 323–325, 1976. View at Google Scholar · View at Scopus
  12. I. Gummelt, H. Hotzel, M. Runge, and H. Kirchoff, “Toxonomic relationship between M. bovis and M. agalactiae,” in Cost 826, Mycoplasmas of Ruminants: Pathogenicity, Diagnostics, Epidemiology and Molecular Genetics, J. Frey and K. Sarris, Eds., pp. 27–29, 1996. View at Google Scholar
  13. J. T. Boothby, D. E. Jasper, and M. H. Rollins, “Characterization of antigens from mycoplasmas of animal origin,” American Journal of Veterinary Research, vol. 44, no. 3, pp. 433–439, 1983. View at Google Scholar · View at Scopus
  14. M. Solsona, M. Lambert, and F. Poumarat, “Genomic, protein homogeneity and antigenic variability of Mycoplasma agalactiae,” Veterinary Microbiology, vol. 50, no. 1-2, pp. 45–58, 1996. View at Google Scholar · View at Scopus
  15. O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall, “Protein measurement with the Folin phenol reagent,” The Journal of Biological Chemistry, vol. 193, no. 1, pp. 265–275, 1951. View at Google Scholar · View at Scopus
  16. V. Bhanuprakash and N. Srivastava, “Partial purification and electrophoretic profile of Mycoplasma arginini antigenic preparations,” Indian Journal of Animal Sciences, vol. 66, no. 6, pp. 542–544, 1996. View at Google Scholar · View at Scopus
  17. G. E. Jones, “Contagious caprine pleuropneumonia,” Technical Series 9, Office of International des Epizootics, Paris, France, 1989. View at Google Scholar
  18. N. C. Srivastava, A. Sikdar, S. K. Srivastava, and P. N. Bhat, “Studies on animal mycoplasmas and development of vaccine against caprine mycoplasma infections,” Annual Report of Division of Bacteriology and Mycology, IVRI, Izatnagar, India, 1986. View at Google Scholar
  19. A. Krogsgaard-Jensen, “Indirect hemagglutination with Mycoplasma antigens: effects of pH on antigen sensitization of tanned fresh and formalinized sheep erythrocytes,” Applied Microbiology, vol. 22, no. 5, pp. 756–759, 1971. View at Google Scholar · View at Scopus
  20. N. C. Srivastava, A. Sikdar, and R. Somvanshi, “Monitoring of serological response in goat given killed M. Capri vaccine by IHA,” Indian Journal of Animal Sciences, vol. 66, pp. 1037–1038, 1992. View at Google Scholar
  21. U. K. Laemmli, “Cleavage of structural proteins during the assembly of the head of bacteriophage T4,” Nature, vol. 227, no. 5259, pp. 680–685, 1970. View at Publisher · View at Google Scholar · View at Scopus
  22. Y. Mori, T. Hamaoka, S. Sato, and S. Takeuchi, “Immunoblotting analysis of antibody response in swine experimentally inoculated with Mycoplasma hyopneumoniae,” Veterinary Immunology and Immunopathology, vol. 19, no. 3-4, pp. 239–250, 1988. View at Google Scholar · View at Scopus
  23. H. Towbin, T. Staehelin, and J. Gordon, “Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications,” Proceedings of the National Academy of Sciences of the United States of America, vol. 76, no. 9, pp. 4350–4354, 1979. View at Google Scholar · View at Scopus
  24. A. Kumar, N. C. Srivastava, and V. P. Singh, “Analysis of Mycoplasma agalactiae and Mycoplasma bovis antigens by Polyacrylamide gel electrophoresis (PAGE),” Indian Journal of Small Ruminants, vol. 16, no. 1, pp. 271–273, 2010. View at Google Scholar
  25. D. Bergonier, M. Solsana, J. Frey, R. Miserez, J. Nicolet, and F. Poumarat, “PCR on16SrRNA gene, REA and SDS-PAGE patterns of M. agalactiae isolates,” in Cost 826, Mycoplasmas of Ruminants: Pathogenicity, Diagnostics, Epidemiology and Molecular Genetics, J. Frey and K. Sarris, Eds., pp. 91–92, 1996. View at Google Scholar
  26. K. Sachse, C. Grajetzki, H. Pfützner, and R. Hass, “Comparison of Mycoplasma bovis strains based on SDS-PAGE and immunoblot protein patterns,” Journal of Veterinary Medicine B, vol. 39, no. 4, pp. 246–252, 1992. View at Google Scholar · View at Scopus
  27. F. Poumarat, M. Solsona, and M. Boldini, “Genomic, protein and antigenic variability of Mycoplasma bovis,” Veterinary Microbiology, vol. 40, no. 3-4, pp. 305–321, 1994. View at Publisher · View at Google Scholar · View at Scopus
  28. S. S. Kannan, Restriction pattern and protein profile of M. mycoides group [M.S. thesis], Deemed University, IVRI, Izatnagar, India, 1998.
  29. H. Andersen, S. Birkelund, G. Christiansen, and E. A. Freundt, “Electrophoretic analysis of proteins from Mycoplasma hominis strains detected by SDS-PAGE, two-dimensional gel electrophoresis and immunoblotting,” Journal of General Microbiology, vol. 133, no. 1, pp. 181–191, 1987. View at Google Scholar · View at Scopus
  30. A. Kumar, N. C. Srivastava, and V. P. Singh, “Antigenic characterization of Mycoplasma agalactiae by SDS-PAGE and Immunoblotting,” Research Journal of Microbiology, vol. 9, no. 1, pp. 59–65, 2014. View at Google Scholar
  31. R. Rosengarten, A. Stetefeld, M. Ahrens, A. Behrens, M. Heller, and H. Kirchhoff, “Protein and antigenic variability of M. bovis,” in Proceedings of the 9th International Congress of International Organization of Mycoplasmology, IOM Letters. No. 2., p. 129, 1992.
  32. A. Kumar, N. C. Srivastava, and V. P. Singh, “Identification of potential diagnostic and protective antigen for Mycoplasma bovis,” Indian Journal of Comparative Microbiology and Infectious Diseases, vol. 34, no. 1, pp. 29–32, 2013. View at Google Scholar
  33. R. H. Leach, “Further studies on classification of bovine strains of mycoplasmatales, with proposals for new species, Acholeplasma modicum and Mycoplasma alkalescens,” Journal of General Microbiology, vol. 75, no. 1, pp. 135–153, 1973. View at Google Scholar · View at Scopus